Identification of Melon CmRDR Gene Family and Its Expression Analysis on CGMMV Infection
RNA dependent RNA polymerases(RDRs)are the key proteins in the RNA silencing pathway.They are responsible for the synthesis of double stranded ribonucleic acids(dsRNAs)using single stranded ribonucleic acid(ssRNA)as template,thus initiating a new round of RNA silencing.It is well known that RDR genes play very im-portant roles in response to plant virus infection.However,RDR genes in melon has not been reported.Therefore,in this study,seven CmRDR genes in melon were identified at the genome-wide level by bioinformatics,and their protein and gene structure,subcellular localization and evolutionary relationship were predicted and analyzed.The cis-acting elements involved in stress response and hormone response were also analyzed.In addition,the expres-sion patterns of 7 CmRDR genes in melon were analyzed via RT-qPCR.The results showed that most CmRDR genes were significantly repressed by Cucumber green mottle mosaic virus(CGMMV)infection.This study was the first to identify RDRs gene family in melon and preliminarily revealed the roles of CmRDR gene in CGMMV in-fection,which provided an effective basis for disease resistance breeding in melon.
MelonRNA-dependent RNA polymerasesCucumber green mottle mosaic virus(CGMMV)Gene expressionPhylogenetic analysis
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