油茶Aux/IAA基因家族的鉴定与分析
Identification and Analysis of AUX/IAA Gene Family in Camellia oleifera
张廷忠 1梁康 1陈荟 1朱亚艳 1许杰1
作者信息
- 1. 贵州省林业科学研究院,贵阳,550005
- 折叠
摘要
为了获取油茶Aux/IAA基因家族的分布和结构特征,利用HMM进行油茶全基因组Aux/IAA基因的鉴定.结果表明,油茶Aux/IAA基因非均匀分布在10条染色体上;序列长度和外显子数量差异较大,氨基酸长度范围在132～1 085之间,外显子数量为2～16个,同一系统发育亚组上的基因具有类似的外显子数量和基序结构;油茶Aux/IAA部分基因具备响应生长素的顺式作用元件外,还具备其他激素响应的基序和光响应元件.转录组数据表明,CoIAA Chr12_3、CoIAA Chr7_5、CoIAA Chr9_3、CoIAA Chr9_4、CoIAA Chr7_6 基因在油茶花芽、花瓣、萼片、雄蕊、雌蕊中高表达,可能与花的形成和发育相关.CoIAA Chr12_5在油茶种子中高表达,可能与油茶种子发育相关.本研究为Aux/IAA基因家族在油茶中的系统研究提供了基础.
Abstract
In order to obtain the distribution and structural characteristics of Camellia oleifera Aux/IAA gene family,HMM was used to identify Aux/IAA genes in the whole genome of C.oleifera.The results showed that C.oleifera Aux/IAA genes were unevenly distributed in 10 chromosomes;the sequence length and the number of exons vary greatly,the amino acid length ranged from 132 to 1 085,the number of exons was 2 to 16,genes on the same phylo-genetic subgroup had similar exon numbers and motif structures;some Aux/IAA genes of C.oleifera had cis-acting elements in response to auxin,as well as other hormone-responsive motifs and light-responsive elements.Transcrip-tome data showed that CoIAA Chr12_3,CoIAA Chr7_5,CoIAA Chr9_3,CoIAA Chr9_4,CoIAA Chr7_6 genes were highly expressed in C.oleifera flower buds,petals,sepals,stamens,and pistils,which were related to flower forma-tion and development.CoIAA Chr12_5 was highly expressed in seeds,which was associated with seed development.This study provided the basis for the systematic study of Aux/IAA gene family in C.oleifera.
关键词
油茶/基因家族/Aux/IAA/启动子元件分析/基因表达Key words
Camellia oleifera/Gene family/Aux/IAA/Promoter element analysis/Gene expression引用本文复制引用
基金项目
贵州本地油茶良种选育项目(特林研2020-15)
贵州省油茶团队服务企业行动计划(黔科合服企[2018]4003)
贵州省油茶工程技术研究中心项目(黔科合平台人才[2018]5252)
出版年
2024
NETL
NSTL
万方数据