首页|油茶Aux/IAA基因家族的鉴定与分析

油茶Aux/IAA基因家族的鉴定与分析

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为了获取油茶Aux/IAA基因家族的分布和结构特征,利用HMM进行油茶全基因组Aux/IAA基因的鉴定.结果表明,油茶Aux/IAA基因非均匀分布在10条染色体上;序列长度和外显子数量差异较大,氨基酸长度范围在132~1 085之间,外显子数量为2~16个,同一系统发育亚组上的基因具有类似的外显子数量和基序结构;油茶Aux/IAA部分基因具备响应生长素的顺式作用元件外,还具备其他激素响应的基序和光响应元件.转录组数据表明,CoIAA Chr12_3、CoIAA Chr7_5、CoIAA Chr9_3、CoIAA Chr9_4、CoIAA Chr7_6 基因在油茶花芽、花瓣、萼片、雄蕊、雌蕊中高表达,可能与花的形成和发育相关.CoIAA Chr12_5在油茶种子中高表达,可能与油茶种子发育相关.本研究为Aux/IAA基因家族在油茶中的系统研究提供了基础.
Identification and Analysis of AUX/IAA Gene Family in Camellia oleifera
In order to obtain the distribution and structural characteristics of Camellia oleifera Aux/IAA gene family,HMM was used to identify Aux/IAA genes in the whole genome of C.oleifera.The results showed that C.oleifera Aux/IAA genes were unevenly distributed in 10 chromosomes;the sequence length and the number of exons vary greatly,the amino acid length ranged from 132 to 1 085,the number of exons was 2 to 16,genes on the same phylo-genetic subgroup had similar exon numbers and motif structures;some Aux/IAA genes of C.oleifera had cis-acting elements in response to auxin,as well as other hormone-responsive motifs and light-responsive elements.Transcrip-tome data showed that CoIAA Chr12_3,CoIAA Chr7_5,CoIAA Chr9_3,CoIAA Chr9_4,CoIAA Chr7_6 genes were highly expressed in C.oleifera flower buds,petals,sepals,stamens,and pistils,which were related to flower forma-tion and development.CoIAA Chr12_5 was highly expressed in seeds,which was associated with seed development.This study provided the basis for the systematic study of Aux/IAA gene family in C.oleifera.

Camellia oleiferaGene familyAux/IAAPromoter element analysisGene expression

张廷忠、梁康、陈荟、朱亚艳、许杰

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贵州省林业科学研究院,贵阳,550005

油茶 基因家族 Aux/IAA 启动子元件分析 基因表达

贵州本地油茶良种选育项目贵州省油茶团队服务企业行动计划贵州省油茶工程技术研究中心项目

特林研2020-15黔科合服企[2018]4003黔科合平台人才[2018]5252

2024

分子植物育种
海南省生物工程协会

分子植物育种

CSTPCD北大核心
影响因子:0.765
ISSN:1672-416X
年,卷(期):2024.22(9)
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