摘要
为探究巴戟天中参与生长发育及次生代谢产物合成的bHLH转录因子,本研究基于巴戟天的转录组数据,对巴戟天的bHLH转录因子进行筛选鉴定、亚细胞定位、保守结构域、GO功能注释、系统进化树及组织特异性表达差异分析.结果显示,鉴定出的90个巴戟天bHLH转录因子的保守结构域大约有60个氨基酸组成,包含2个不同的功能结构区,其中83个bHLH转录因子具有识别并结合E-box的能力.63个bHLH转录因子定位于细胞核中,27个位于细胞外基质.GO功能注释分类结果显示与生物过程、分子功能和细胞成分有关的GO terms数量分别为39、109和61个.系统进化树分析结果可知,除S2、S4、S12、S14、S17和S20亚家族外,90个巴戟天bHLH转录因子在其他亚家族均有分布.RT-qPCR结果验证了部分MobHLH转录因子在巴戟天不同组织中的表达模式.鉴定的90个巴戟天bHLH转录因子为后续进一步研究巴戟天bHLH转录因子家族提供了一定的理论基础.
Abstract
To explore the bHLH transcription factors related to development and secondary metabolite biosynthe-sis in Morinda officinalis,this study was conducted to screen and identify the MobHLH transcription factors(TFs)based on the transcriptome data.The cellular localization,conserved structural domains,GO functional annotation,phylogenetic tree,and tissue-specific expression differences were analyzed.The results showed that the 90 identi-fied MobHLH TFs had a conserved structural domain consisting of approximately 60 amino acids and contained two different functional structural regions,of which 83 MobHLH TFs had the ability to recognize and bind E-box.63 MobHLH TFs were localized in the nucleus and 27 were located in the extracellular.The results of GO func-tional annotation showed that 39,109,and 61 GO terms were classified into biological processes,molecular func-tions,and cellular components,respectively.Phylogenetic tree analysis showed that 90 MobHLH TFs were dis-tributed in all subfamilies except subfamilies S2,S4,S12,S14,S17,and S20.RT-qPCR results verified the expres-sion patterns of some MobHLH TFs in different tissues of M.officinalis.The identified 90 MobHLH TFs provided a theoretical basis for further study of the bHLH TFs in M.officinalis.
基金项目
福建省科技创新平台项目(2008Y2001)
福建农林大学科技发展创新基金(CXZX2017497)
NETL
NSTL
万方数据