Identification and Analysis of the bHLH Transcription Factor Based on RNA-seq Database in Morinda officinalis
To explore the bHLH transcription factors related to development and secondary metabolite biosynthe-sis in Morinda officinalis,this study was conducted to screen and identify the MobHLH transcription factors(TFs)based on the transcriptome data.The cellular localization,conserved structural domains,GO functional annotation,phylogenetic tree,and tissue-specific expression differences were analyzed.The results showed that the 90 identi-fied MobHLH TFs had a conserved structural domain consisting of approximately 60 amino acids and contained two different functional structural regions,of which 83 MobHLH TFs had the ability to recognize and bind E-box.63 MobHLH TFs were localized in the nucleus and 27 were located in the extracellular.The results of GO func-tional annotation showed that 39,109,and 61 GO terms were classified into biological processes,molecular func-tions,and cellular components,respectively.Phylogenetic tree analysis showed that 90 MobHLH TFs were dis-tributed in all subfamilies except subfamilies S2,S4,S12,S14,S17,and S20.RT-qPCR results verified the expres-sion patterns of some MobHLH TFs in different tissues of M.officinalis.The identified 90 MobHLH TFs provided a theoretical basis for further study of the bHLH TFs in M.officinalis.
NETL
NSTL
万方数据
