Identification and Expression Analysis of Telomerase Reverse Transcriptase Gene(PeTERT)from Phyllostachys edulis
Telomerase is essential for maintaining telomere integrity.Telomerase reverse.transcriptase(TERT)is the core component of telomerase,which lengthens telomeres mainly by synthesizing telomere DNA to maintain telomere integrity.At present,there have been no reports on the PeTERT gene of Phyllostachys edulis.In this study,bioinformatics analysis was carried out on PeTERT,and RT-qPCR was used to detect the PeTERT expres-sion patterns in leaf,stem,root and callus and seedlings treated with exogenous hormones and abiotic stress.The results showed that the PeTERT gene sequence was identified in Phyllostachys edulis genome.The CDS sequence is 3 789 bp,PeTERT contains 1 262 amino acids,the isoelectric point is 9.45,the relative molecular mass is 143.74 kD,showing the basic characteristics of the secondary and tertiary structures of TERT with TRBD and RT conservative domain structure.The PeTERT promoter sequence contains multiple hormone and abiotic stress re-sponse elements.RT-qPCR detected the expression of PeTERT in Phyllostachys edulis,and the expression level in callus with strong cell division ability was 2.5～5 times higher than that in roots,stems and leaves of seedlings.The exogenous hormones ABA,MeJA,GA3 and SA could affect the expression of PeTER Tin seedlings,and PetERT could also respond to stress conditions such as low temperature(4 ℃),high temperature(42 ℃),high salt(200 mmol/L),and drought(20％PEG-6000),indicating that PeTERT may exert non-telomere function.This research provides im-portant reference for studying the biological function of PeTERT gene.
NETL
NSTL
万方数据
