A KASP-Based Rapid Procedure for Bottle Gourd Hybrid F1 Seed Purity Survey
Bottle gourd is one of the important distinctive vegetables in China.At present,there are many varieties of bottle gourds in the market,and disputes of the same name,different names of the same thing and intellectual property rights often occur.Traditional identification of varieties mainly depends on low efficiency field planting.In this study,'Zhepu No.9',a new variety with high-quality and short straight fruit shape,and its parents were used as test materials,and 3 pairs of parent-specific SNP primers of'Zhepu No.9'were selected based on LGC KASP high-throughput technology platform.The results showed that the purity of F1 seeds of'Zhepu No.9'was 97.8％,which was consistent with the field purity identification.The method has advantages of good accuracy,low cost and high efficiency.The expense of single hole does not exceed 1 RMB,and the whole detection procedure only takes about 5 days,which greatly shortens the time for variety identification.This study provided a precise,rapid and convenient KASP-based procedure for bottle gourd seed purity survey with a broad application prospect.
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