基于KASP标记快速鉴定瓠瓜杂种F1纯度的方法
A KASP-Based Rapid Procedure for Bottle Gourd Hybrid F1 Seed Purity Survey
冯子珊 1吴晓花 2李艳伟 2鲁忠富 2王尖 2吴新义 2汪宝根 2叶子弘 1李国景 2汪颖2
作者信息
- 1. 中国计量大学生命科学学院,杭州,310018
- 2. 浙江省农业科学院蔬菜研究所,杭州,310021
- 折叠
摘要
瓠瓜为中国南方重要特色瓜类蔬菜之一,随着瓠瓜育成品种数量逐年增多,同名异种、同种异名引发的知识产权纠纷多见.传统上对品种的鉴定主要依靠田间种植,效率低.本研究以浙江省农业科学院蔬菜所新育成的高品质短棒瓠瓜杂交种'浙蒲9号'及其亲本为试材,选取了 3对'浙蒲9号'亲本特异性SNP引物,基于LGC KASP高通量技术平台,对'浙蒲9号'父本、母本以及92份F1材料进行鉴定,结果显示'浙蒲9号'F1种子纯度为97.8％,与田间种植纯度鉴定结果一致.该方法准确性好、成本低且高效,单孔不超过1元,检测全程仅需5 d左右,大大缩短了品种鉴定的时间,是一种准确、简便、快速的瓠瓜品种鉴定和纯度检测方法,应用前景非常广泛.
Abstract
Bottle gourd is one of the important distinctive vegetables in China.At present,there are many varieties of bottle gourds in the market,and disputes of the same name,different names of the same thing and intellectual property rights often occur.Traditional identification of varieties mainly depends on low efficiency field planting.In this study,'Zhepu No.9',a new variety with high-quality and short straight fruit shape,and its parents were used as test materials,and 3 pairs of parent-specific SNP primers of'Zhepu No.9'were selected based on LGC KASP high-throughput technology platform.The results showed that the purity of F1 seeds of'Zhepu No.9'was 97.8％,which was consistent with the field purity identification.The method has advantages of good accuracy,low cost and high efficiency.The expense of single hole does not exceed 1 RMB,and the whole detection procedure only takes about 5 days,which greatly shortens the time for variety identification.This study provided a precise,rapid and convenient KASP-based procedure for bottle gourd seed purity survey with a broad application prospect.
关键词
瓠瓜/KASP标记/纯度鉴定/快速/浙蒲9号Key words
Bottle gourd/KASP marker/Purity survey/Rapid/Zhepu No.9引用本文复制引用
基金项目
浙江省基础公益研究计划(LGN19C150011)
浙江省蔬菜新品种选育科技重大专项(十四五)(2021C0-2065)
瓠瓜高质量种子生产关键技术研究项目(2021C0204-2)
出版年
2024
NETL
NSTL
万方数据