SNP Analysis of Mango Bacterial Black Spot Resistance Disease Based on Re-sequencing
Mango bacterial black spot disease is one of the important bacterial diseases in mango production.In order to reveal the difference in resistance of mango varieties to bacterial black spot disease,this study used whole-genome resequencing technology to identify highly resistant bacterial black spot disease varieties'Renong No.1'and the highly susceptible bacterial black spot disease variety'Keitt'were sequenced,and compared with the reference genome'Red Ivory',data statistics and quality detection,sequence comparison and polymorphic SNP lo-cus analysis were performed.The results showed that at a sequencing depth of 10X,'Keitt'and'Renong No.1'ob-tained 5.93 Gb and 4.55 Gb data respectively,and the filtered Q30 reached 92.4％ and 92.3％.Comparing the ob-tained original data with the reference genome,the number of reads in the comparison of'Keitt'and'Renong No.1'are 31 108 817 and 30 217 182,respectively.3 530 706 and 3 325 884 SNP loci were detected in'Keitt'and'Renong No.1',respectively.The number of SNP loci that produced non-synonymous mutations were 133 283 and 127 148,respectively,and the heterozygosity was 0.43％ and 0.36％,the ratio of conversion and transversion is 2.24 and 2.25.In the annotation analysis of gene function,3 258 857 differential SNP loci and 33 161 pieces of gene information were obtained,of which 937 genes are involved in the regulation of disease-resistant proteins.The results of this study can reflect to a certain extent thedifferences in genomic resistance of mango varieties that are resistant to bacterial black spot disease,and can be used for the next step in the resistance evaluation of bacteri-al black spot disease hybrid population materials and the development of specific molecular markers for disease re-sistance,and provide theoretical basis for the creation and breeding of high-quality mango varieties resistant to dis-ease in the later stage.
Whole genome re-sequencingMangoBacterial black spot diseaseSNP
NETL
NSTL
万方数据
