Identification of Seed Purity of Melon Hybrid'Yuguiren'by SSR Molecular Markers
In order to accurately and quickly screen the SSR primers for identifying the purity of melon hybrids,in this study,18 pairs of melon SSR core primers were used to screen the primers and identify the seed purity of mel-on hybrids'Yuguiren'and its parents.The results showed that two pairs of primers(CmS57 and CmS59)had both biparental bands in the offspring and polymorphism among the parents with clear bands and obvious intervals,and were verified in the hybrid population with 97.8％ and 100.0％ purity;the error between the identification results of SSR markers and the field survey results was 1.1％.It showed that the primers CmS57 and CmS59 screened in this study could be used as specific primers for seed purity testing of melon hybrids'Yuguiren',and also indicated that the application of SSR molecular marker technology is feasible for the purity identification of melon seeds.
SSR molecular markerMelonYuguirenPurity identification
NETL
NSTL
万方数据
