Effect of Photoperiod on Hormone Content and Gene Expression in Pear Leaves
Photoperiod refers to the relative length of day and night in a day,widely regulating many aspects of plant growth and development.Studying the diurnal variation of endogenous hormones in pear leaves under different photoperiod conditions and exploring the molecular mechanism of endogenous hormones in response to photoperiod induction can provide theoretical basis for synergistic use of photoperiod and hormones to regulate plant growth and development.In this study,pear seedlings growing under long-day and short-day conditions were sampled at different time points throughout the day.The contents of five endogenous plant hormones,such as auxin,cytokinin,abscisic acid,ethylene and jasmonic acid,and the expression patterns of related genes in pear leaves were analyzed.It was shown that the five hormones in pear leaves showed different diurnal dynamic patterns.The content of hormone changed significantly under two photoperiod conditions.Through the GO analysis of differentially expressed genes in the transcriptome,several hormone-related biological processes were significantly enriched.For example,upregulated differentially expressed genes were mainly enriched in auxin transport processes.Differential gene expression patterns related to hormone biosynthesis,transport and signal transduction were analyzed,and candidate key genes responding to photoperiodic induction were screened.These results provide a reference for further investigation of photoperiod regulation of pear hormone response.
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