Rapid Propagation Technology of Eucommia ulmoides Aseptic Seedling Tis-sue Culture
Eucommia ulmoides is a special precious tree species in China,which has important medical and eco-nomic value.Aiming at the problems of easy pollution,difficult induction of adventitious buds and roots in E.ul-moides tissue culture,aseptic seedlings were used as materials to study the optimal explants,the optimal basic medium for adventitious bud induction,proliferation culture and rooting promotion,the type and concentration of plant growth regulators.The results showed that with the shoot tip of E.ulmoides aseptic seedling as explants,MS as the basic medium,0.5 mg/L or 1.0 mg/L 6-BA and 0.5 mg/L NAA were the most suitable concentration for cal-lus induction;although the callus induction rate was low when 0.5 mg/L 6-BA was used alone,the adventitious bud induction rate was the highest,88.9％,which was suitable for subsequent adventitious bud proliferation exper-iments.With WPM as the basic medium,adding 1.0 mg/L IBA was the most suitable rooting medium,the rooting rate was 88.9％,and the root system was longer.The research results will provide technical reference for tissue culture and industrial technology,genetic engineering of E.ulmoides.
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