Analysis on the Changes of Tobacco Pollen Inclutions and Pollen Vitality in Drying Process
In order to explore the physiological mechanism of vitality changes of tobacco pollen during the drying process,fresh anthers of variety'K326'was used as the materials to analyze the changes of pollen in vitality,water content,inclusions,antioxidant enzymes,MDA and other physiological indicators during the drying process.The re-sults showed that the pollen vitality and water content decreased rapidly with the extension of drying time,and then stabilized.The content of soluble sugar and soluble starch showed a trend of decline-increasing-decrease-stable.The content of soluble protein first decreased and then tended to stable while the total amino acid content first increases and then tends to be stable,and their changes are opposite.The proline content presented a rising-decreasing-slowly rising trend and the ATPase activity first decreased and then tended to be stable;SOD,POD and CAT activities in-creased first and then decreased slowly;MDA content had been slowly rising.Water content and ATPase activity are the two most important factors affecting pollen vitality,and these two indicators can provide important references for tobacco pollen drying and storage.
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