摘要
为探究不同土壤水分条件下滇黄精块茎多糖积累的规律及其酶活性变化特征,本研究采用盆栽控水法,对3年生滇黄精植株进行4种水分胁迫处理,并对部分重度干旱植株进行复水处理;采用分光光度计法测定SS、SPS、AI、UGP、NI5种酶的活性;采用0.2％蒽酮-硫酸比色法测定多糖含量.结果显示,SS、SPS、AI、UGP、NI分别在含水量在20％、40％、20％、20％、20％时酶活性达到峰值;滇黄精多糖含量在20％含水量处理组最高.干旱胁迫与多糖含量的相关性分析发现,土壤含水量与多糖含量呈显著负相关.本研究表明中度和重度干旱可以提高滇黄精多糖合成相关酶的活性,且有利于滇黄精块茎多糖的积累,能有效提高滇黄精品质,这对滇黄精的种植有十分重要的指导意义.
Abstract
In order to investigate the accumulation of polysaccharide and the changes of enzyme activities in tuber of P.kingianum under different soil moisture conditions,this study used pot cultivation method to control water stress on 3-year old plants under four kinds of water stress treatments and some severely drought plants were rewa-tered.The activities of SS,SPS,AI,UGP and NI were determined by spectrophotometer.The content of polysaccha-ride was determined by 0.2％anthrone-sulfuric acid colorimetry.The results showed that the enzyme activity of SS,SPS,AI,UGP and Ni reached the peak when the water content was 20％,40％,20％,20％,and 20％,respectively.The highest polysaccharide content was found in 20％water content group.Correlation analysis between drought stress and polysaccharide content showed that there was a significant negative correlation between soil moisture and polysaccharide content.This study showed that moderate and severe drought could improve the activities of poly-saccharide biosynthesis related enzymes,and was beneficial to the accumulation of polysaccharide from the tuber of P.kingianum,which could effectively improve the quality of P.kingianum,which had a very important guiding sig-nificance for the cultivation of P.kingianum.
基金项目
国家自然科学基金(31760347)
云南省创新人才培养项目(2018HB085)
云南中医药大学高层次人才科学研究项目(30270104868)
云南省科技厅-云南中医药大学中医联合专项重点项目(202001AZ070001-010)
NETL
NSTL
万方数据