Effects of Drought Stress on Polysaccharides and Metabolic Enzymes of Polygonatum kingianum
In order to investigate the accumulation of polysaccharide and the changes of enzyme activities in tuber of P.kingianum under different soil moisture conditions,this study used pot cultivation method to control water stress on 3-year old plants under four kinds of water stress treatments and some severely drought plants were rewa-tered.The activities of SS,SPS,AI,UGP and NI were determined by spectrophotometer.The content of polysaccha-ride was determined by 0.2％anthrone-sulfuric acid colorimetry.The results showed that the enzyme activity of SS,SPS,AI,UGP and Ni reached the peak when the water content was 20％,40％,20％,20％,and 20％,respectively.The highest polysaccharide content was found in 20％water content group.Correlation analysis between drought stress and polysaccharide content showed that there was a significant negative correlation between soil moisture and polysaccharide content.This study showed that moderate and severe drought could improve the activities of poly-saccharide biosynthesis related enzymes,and was beneficial to the accumulation of polysaccharide from the tuber of P.kingianum,which could effectively improve the quality of P.kingianum,which had a very important guiding sig-nificance for the cultivation of P.kingianum.
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