Localization of Melon Wilt Resistance-related Genes Based on BSA-seq and Annotation of Candidate Genes
To explore the genes related to melon blight resistance,this study was conducted to construct an F2 population with YN,a high blight resistance self-inbred line,as the mother and CG,a high blight susceptible self-inbred line,as the father,and to construct extreme high resistance and high susceptibility hybrid pools based on the F2 population,and whole genome resequencing with 30 x coverage depth was carried out for the offspring hybrid pools and the biparental pools,by calculating the SNP-index of the two offspring pools,comparing the dif-ferences in SNP-index between the highly resistant and highly susceptible pools,locating the association regions with disease resistance,and predicting candidate genes based on gene annotation information.The results showed that the SNP loci and IndeLs obtained from the four samples ranged from 910 000 to 2.5 million and 170 000 to 450 thousand,among which 35 000 to 94 thousand and 1 802 to 4 774 were located in the exon region,respective-ly.Based on SNP-index association analysis,the candidate region was localized to 1 interval of melon chromo-some Chr09 with a total length of 1.37 Mb in the interval of 1 to 1 370 000 bp containing 197 genes and 7 442 SNP-indel loci at 99％confidence interval.By KEGG and GO database analysis,the candidate genes were annotat-ed into 18 biological processes,9 cellular components and 6 molecular functions Mainly involved inamino acid biosynthesis,amino acid metabolism,ester metabolism and sugar metabolism,etc.According to the analysis,a to-tal of 22 genes related to disease resistance were found in the candidate region,3 were related to F-box genes,3 hydrolytic protein-related genes,four ethylene-responsive transcription factors,one ubiquitin protein ligase,two GDSL esterases,etc.The results of this study will provide new ideas for the molecular mechanism of disease resis-tance of melon wilt,and provide a reference for the development of functional molecular markers for its disease re-sistance and cloning of related genes.
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