Isolation and Identification of Leaf Disease Pathogens from Iris Louisiana Cultivated on Land
The newly introduced iris louisiana was prone to leaf diseases under terrestrial cultivation conditions,which seriously affected its landscape value.In order to provide a scientific basis for the precise prevention and control of terrestrial iris louisiana leaf diseases,a study on the isolation and characterization of terrestrial Iris hybrids'Louisiana'leaf disease pathogens was carried out.Using the leaves of iris louisiana cultivated on land as test materials,we studied and identified the pathogen species of terrestrial iris louisiana leaf diseases by using tissue isolation method,Koch's law for isolation and pathogenicity test of pathogens,as well as by using morphological observation and analysis and molecular biology identification of pathogenic pathogens.The results showed that six species of fungi,YW1 to YW6,were isolated from diseased tissues,and five species were identified as pathogenic fungi(YW1,YW2,YW4,YW5,YW6);among them,YW1 is Fusarium fujikuroi;YW2 and YW6 are Cladosporium cladosporioides and Cladosporium anthropophilum,respectively;YW4 and YW5 are Penicillium expansum and Penicillium crustosum,respectively.The results of this study enriched the pathogens of leaf diseases of iris louisiana.and provided scientific basis for the comprehensive control of leaf diseases of terrestrial iris louisiana
Iris louisianaLeaf diseaseKoch's lawGene sequencingPhylogenetic tree
NETL
NSTL
万方数据
