RAPD Analysis of Genetic Diversity of Hemerocallis fulva Germplasm Resources in Southern Qinling Mountain
In order to investigate Hemerocallis germplasm resources in the south of Qinling Mountain,totally 23 wild Hemerocallis fulva resources from different habitats in Qinling Mountain,23 wild Hemerocallis fulvaresources and the cultivated variety of yellow cabbage'Shayuanhua'from different habitats in the Qinling Mountain were used as test materials.RAPD technology was used to select 11 primers with good polymorphism from 35 random primers for genetic diversity analysis of 24 Hemerocallis germplasm materials.The results showed that a total of 492 amplified sites were obtained from 24 samples,of which 446 were polymorphic bands,accounting for 90.7％,and the polymorphism rate ranged from 80.0％to 96.9％.The genetic distance matrix and UPGMA clustering analysis showed that the genetic distance between the 24 Hemerocallis materials ranged from 0.00～0.69.At the threshold of 0.65,total of 24 Hemerocallis materials could be divided into 3 categories,in which the wild germ-plasm No.23 and the cultivated variety'Shayuanhua'No.24 were clustered into each one category respectively,even though they are all yellow flowers.The wild Hemerocallis samples from other sampling sites were grouped into one category,and the colors range from orange to tangerine.However,there were no obvious regularities between flower diameter,plant height and the habitat condition,which showed great difference in biological traits.On the whole,the Hemerocallis germplasm resources distributed in southern Qinling Mountain had rich genetic diversity whether among interspecies or intraspecies variation,but the inconsistency between the results of cluster analysis and geographical distribution also existed.All these results should have a certain reference significance for the collection,preservation and breeding of Hemerocallis resources and other groundcover germplasm resources in China.
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万方数据
