Analysis of Soil Microbial Composition and Metabolic Pathways of the Av-ocado Wilt Complex Plants
To further study the microbial composition in the soil of root zone of avocado wilt complex plants,we collected 18 soil samples from the root zone of symptomatic plants and asymptomatic plants from three avocado plantations in tropical areas,with 3 replicates in each group,metagenomic sequencing was performed based on NovaSeq 6000 sequencing platform and the correlation analysis was performed with soil physic-chemical proper-ties.The results showed that:(1)The soil microbial diversity in the root zone of avocado wilt complex plants was higher than that of asymptomatic plants.(2)The abundance of Rhodopseudomonas palustris in the soil of symptomatic plants was significantly lower than that in asymptomatic plants by 47％,which was beneficial for avocado to maintain normal growth.Pseudomonas unclassified was only presented in the soil of symptomatic plants.(3)The content of available phosphorus in the soil of the root zone of symptomatic plants was significantly higher than that of asymp-tomatic plants(P=0.019),and there were no significant differences in pH,soil organic matter,available potassium and alkali nitrogen content between the two groups.The measured soil physicochemical properties were signifi-cantly correlated with multiple soil microbes in root zones.(4)The metabolic pathways such as isoquinoline al-kaloid biosynthesis and type Ⅱ polyketide biosynthesis were shown to associated with the occurrence of avocado wilt complex;on the other hand,pentose phosphate pathway,sesquiterpene and triterpene biosynthesis,furfural degradation and other metabolisms were negatively associated with avocado wilt complex.Avocado wilt com-plex(AWC)is one of the most important diseases in the worldwide avocado industry,which seriously restricts the development of this industry.The interactions between soil microbes and plant roots are directly related to plant health,this study can provide a theoretical basis for exploring the biotic and abiotic factors correlated with avocado wilt complex and can provide the effective guide line for planting of avocado.
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