高温胁迫对两个杉木种源多光谱荧光和抗氧化酶的影响
Effects of High Temperature Stress on Multispectral Fluorescence and An-tioxidant Enzyme in Two Chinese Fir Provenances
金鹏 1池艺 1舒鹏州 1金松恒1
作者信息
- 1. 浙江农林大学林业与生物技术学院,杭州,311300;浙江农林大学暨阳学院园林学院,诸暨,311800
- 折叠
摘要
本研究选择福建三明(FJSM)和江西九江(JXJJ)两地两年生的杉木种源作为试验材料,通过在人工气候箱内进行盆栽试验,研究了不同温度(25 ℃,30 ℃,35 ℃,40 ℃)对两个杉木种源的抗氧化酶活性和多光谱荧光的影响.结果表明:杉木的多光谱荧光参数F440、F520、F690、F740及其比值F690/F740随着高温胁迫的增加而升高.在高温胁迫下,FJSM的F690/F740的升高程度低于JXJJ.F690/F740的升高表明叶绿素含量的下降,FJSM种源在高温胁迫下叶绿素降解速度较慢,对高温的抗性更强.另外,经过高温胁迫后,杉木的SOD、POD和CAT等抗氧化酶活性总体上升.与JXJJ种源相比,FJSM在高温胁迫下抗氧化酶活性上升更大,下降趋势更小,表明FJSM更强的抗高温能力与抗氧化酶密切相关.
Abstract
This study selected two-year old Chinese fir provenances from Sanming in Fujian Province(FJSM)and Jiujiang in Jiangxi Province(JXJJ)as experimental materials,and conducted pot experiments in an artificial cli-mate box to study the effects of different temperatures(25 ℃,30 ℃,35 ℃,40 ℃)on the antioxidant enzyme ac-tivity and multispectral fluorescence of the two Chinese fir provenances.The results showed that the multispectral fluorescence parameters F440,F520,F690,F740 and their ratios F690/F740 of Chinese fir increased with the in-crease of high temperature stress.Under high temperature stress,the elevation of F690/F740 in FJSM was lower than that in JXJJ.The elevation of F690/F740 indicated a decrease in chlorophyll content,the slower chlorophyll degradation rate in FJSM under high temperature stress suggested a stronger resistance to high temperatures.In ad-dition,after high temperature stress,the overall activity of antioxidant enzymes such as SOD,POD,and CAT in Chinese fir increased.Compared with the JXJJ source,FJSM showed a greater increase in antioxidant enzyme ac-tivity and a smaller decrease trend under high temperature stress,indicating that FJSM's stronger high-temperature resistance is closely related to antioxidant enzymes.
关键词
杉木/高温胁迫/多光谱荧光/抗氧化酶Key words
Chinese fir/High temperature stress/Multispectral fluorescence/Antioxidant enzyme引用本文复制引用
基金项目
国家重点研发计划项目(2019YFE0118900)
出版年
2024
NETL
NSTL
万方数据