低温处理对抗寒差异侧钝叶草光合性能的影响
Effects of Photosynthetic Performance of Cold Resistant Differential Steno-taphrum secundatum on Low Temperature Treatment
顾慕荣 1王志勇 2李玲 3陈静波 3郝江珊 4杨虎彪 5郭海林 3宗俊勤 3廖丽1
作者信息
- 1. 海南大学热带作物学院,海口,570228
- 2. 海南大学林学院,热带特色林木花卉遗传与种质创新教育部重点实验室,海口,570228
- 3. 江苏省中国科学院植物研究所(南京中山植物园),南京,210014
- 4. 金华职业技术学院,金华,321007
- 5. 中国热带农业科学院热带作物品种资源研究所,农业部华南作物基因资源与种质创制重点实验室,海口,571101
- 折叠
摘要
本研究以2份抗寒性具有显著差异的侧钝叶草(Stenotaphrum secundatum)品系S13(抗寒材料)与S55(敏感材料)进行模拟低温处理,在0(CK)、3、7、14d测定其光合性能,对其光合差异进行分析.结果表明,随着低温胁迫时间的延长,两份材料的净光合速率(Pn)、气孔导度(Gs)和蒸腾速率(Tr)均显著(P＜0.05)下降,而S13胞间CO2浓度(Ci)呈先显著(P＜0.05)降后显著(P＜0.05)升的趋势,S55前期平稳后期显著(P＜0.05)上升,说明低温胁迫前期是由于气孔限制因素引起的侧钝叶草Pn下降,后期是非气孔因素;叶绿素含量(Chl)、类胡萝卜素含量(Car)和叶绿素荧光参数(Fv/Fm)均呈逐步下降趋势,说明侧钝叶草受到光抑制.两份材料规律基本一致,但对低温胁迫的反应存在显著差异(P＜0.05).低温处理3 d后,S13的Chl、Car和Fv/Fm显著(P＜0.05)高于S55;低温处理7d后,S13的Pn、Gs、Tr、Chl、Car和Fv/Fm均显著(P＜0.05)高于S55;低温处理14 d后,S13的Pn、Chl和Car仍显著(P＜0.05)高于S55,其他指标差异不显著(P＞0.05).本试验通过研究低温处理对不同侧钝叶草材料光合特性的影响,为钝叶草抗寒栽培和育种研究提供参考.
Abstract
Two strains of Stenotaphrum secundatum,which were cold resistant material S13 and sensitive material S55 based on previous studies,were analyzed on the differences in photosynthesis at 0,3,7 and 14 d through simu-lated low temperature treatment.The results showed that the net photosynthetic rate(Pn),stomatal conductance(Gs)and transpiration rate(Tr)of the two materials decreased significantly with the extension of cold stress time,while the intercellular CO2 concentration(Ci)rised after the first drop,indicating that the Pn of S.secundatum decreased due to stomatal limitation factors in the early stage of cold stress,but not due to stomatal factors in the later stage.The chlorophyll content(Chl),carotenoids content(Car)and chlorophyll fluorescence parameters(Fv/Fm)showed a gradual decreasing trend,which indicated that S.secundatum was photoinhibition.After 3 days of low tempera-ture treatment,the Chl,Car and Fv/Fm of S13 were significantly higher than those of S55,but there was no signifi-cant difference in photosynthetic parameters.After 7 days of low temperature treatment,Pn,Gs,Tr,Chl,Car and Fv/Fm of S13 were significantly higher than those of S5 5,and Ci was lower than that of S5 5.After 14 days of low tem-perature treatment,Pn,Chl and Car of S13 were still significantly higher than those of S55,Ci was lower than that of S55,and there was no significant difference in other indexes.This experiment was conducted to study the ef-fects of low temperature treatment on photosynthetic characteristics of different S.secundatum,so as to provide reference for the cold-resistant cultivation and breeding of S.secundatum.
关键词
低温/侧钝叶草/光合色素/光合参数/最大光化学效率Key words
Low temperature/Stenotaphrum secundatum/Photosynthetic pigment/Photosynthetic parameters/Maximal photochemistry efficiency引用本文复制引用
基金项目
中国热带农业科学院科技基础资源调查专项(2017FY100601)
国家自然科学基金项目(31560564)
句容市科技计划项目(ZA32104)
句容市科技计划项目(ZB22101)
金华市重大(重点)科技计划项目(2022-2-023)
海南省研究生创新科研课题(Qhys2021-176)
出版年
2024
NETL
NSTL
万方数据