Cloning and Expression Analysis of Soybean GmbZIP27 Gene
The bZIP proteins are a class of transcription factors that regulate plant growth and resistance.In order to explore the biological function of bZIP in soybean,a bZIP gene GmbZIP27 was cloned from soybean by PCR,and the bioinformatics,expression pattern and binding specificity of the gene were analyzed.The results showed that GmbZIP27 was an unstable hydrophobic protein with an open reading frame length of 825 bp,encoding 274 amino acids,a molecular weight of 30.426 kD,and an isoelectric point of 8.54.It contained active sites of histidine and serine,and its secondary structure was dominated by α-helix and β-fold.Subcellular localization analysis revealed that GmbZIP27 was located in the nucleus.Yeast one-hybrid showed that GmbZIP27 could not bind to the ABRE element in the promoter GmFBX176.Expression pattern analysis showed that GmbZIP27 could be expressed in all the detected tissues,and the expression level was successively from high to low in cotyledon,root,leaf and stem,indicating that GmbZIP27 might be involved in the regulation of soybean growth and development.These study provide a theoretical basis for further analysis of the functions of bZIP transcription factors in soybean development and abiotic stress response.
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