木薯MeCYP79D1和MeCYP79D2基因启动子克隆及表达模式分析
Cloning and Expression Pattern Analysis of MeCYP79D1 and MeCYP79D2 Promoters from Cassava
王昕 1符东青 2李瑞梅 3刘姣 3郭建春 3胡新文 4姚远 3王亚杰3
作者信息
- 1. 海南大学生命科学学院,海口,570228;中国热带农业科学院热带生物技术研究所,海南热带农业资源研究院,农业农村部热带作物生物学与遗传资源利用重点实验室,海南省热带农业生物资源保护与利用重点实验室,海口,571101
- 2. 中国热带农业科学院热带生物技术研究所,海南热带农业资源研究院,农业农村部热带作物生物学与遗传资源利用重点实验室,海南省热带农业生物资源保护与利用重点实验室,海口,571101;海南大学热带作物学院,海口,570228
- 3. 中国热带农业科学院热带生物技术研究所,海南热带农业资源研究院,农业农村部热带作物生物学与遗传资源利用重点实验室,海南省热带农业生物资源保护与利用重点实验室,海口,571101
- 4. 海南大学生命科学学院,海口,570228
- 折叠
摘要
MeCYP79D1和MeCYP79D2基因是调控木薯生氰糖苷合成的关键基因,研究其表达特性对低氰木薯改良具有重要意义.为了探究MeCYP79D1和MeCYP79D2基因在SC8木薯品种中的表达模式,本研究克隆获得了 SC8木薯品种的MeCYP79D1和MeCYP79D2基因启动子,发现它们的启动子上具有ABA、SA、GA、MeJA及生长素等激素响应元件,同时具有低温、干旱、伤害胁迫相关元件.采用ABA、SA、GA3、MeJA、低温处理,证明MeCYP79D1和MeCYP79D2基因表达受激素和低温调控.MeCYP79D1和MeCYP79D2基因主要在须根中表达,其次为嫩叶;在块根发育过程中,MeCYP79D1和MeCYP79D2基因主要在块根形成期和膨大前期表达.此研究结果为解析MeCYP79D1和MeCYP79D2基因表达调控机理提供了新的线索.
Abstract
MeCYP79D1 and MeCYP79D2 genes are key genes regulating the synthesis of cyanogenic glycosides in cassava.It is of great significance to study their expression characteristics for the improvement of low cyanide cassava.To explore the functions of MeCYP79D1 and MeCYP79D2 gene in SC8 cassava varieties,the Me-CYP79D1 and MeCYP79D2 gene promoters of SC8 were cloned in this study.It was found that their promoters had hormone response elements such as ABA,SA,GA,MeJA and auxin,as well as low temperature,drought and injury stress related elements.The expression of MeCYP79D1 and MeCYP79D2 was regulated by hormones and low temperature by ABA,SA,GA3,MeJA and low temperature treatment.MeCYP79D1 and MeCYP79D2 genes were mainly expressed in fibrous roots,followed by young leaves;during root development,MeCYP79D1 and MeCYP79D2 genes were mainly expressed in the root formation stage and the early expansion stage.The results of this study provide new clues for analyzing the regulation mechanism of MeCYP79D1 and MeCYP79D2 gene ex-pression.
关键词
木薯(Manihot/esculenta)/启动子克隆/基因表达/生氰糖苷Key words
Cassava/Promoter cloning/Gene expression/Cyanogenic glucoside引用本文复制引用
基金项目
海南省科技专项(ZDKJ2021012)
现代农业产业技术体系建设专项(CARS-11-HNGJC)
出版年
2024
NETL
NSTL
万方数据