Cloning and Expression Pattern Analysis of MeCYP79D1 and MeCYP79D2 Promoters from Cassava
MeCYP79D1 and MeCYP79D2 genes are key genes regulating the synthesis of cyanogenic glycosides in cassava.It is of great significance to study their expression characteristics for the improvement of low cyanide cassava.To explore the functions of MeCYP79D1 and MeCYP79D2 gene in SC8 cassava varieties,the Me-CYP79D1 and MeCYP79D2 gene promoters of SC8 were cloned in this study.It was found that their promoters had hormone response elements such as ABA,SA,GA,MeJA and auxin,as well as low temperature,drought and injury stress related elements.The expression of MeCYP79D1 and MeCYP79D2 was regulated by hormones and low temperature by ABA,SA,GA3,MeJA and low temperature treatment.MeCYP79D1 and MeCYP79D2 genes were mainly expressed in fibrous roots,followed by young leaves;during root development,MeCYP79D1 and MeCYP79D2 genes were mainly expressed in the root formation stage and the early expansion stage.The results of this study provide new clues for analyzing the regulation mechanism of MeCYP79D1 and MeCYP79D2 gene ex-pression.
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