Transcriptome Analysis of the Effect of Exogenous Calcium Ions on Straw-berry Seedlings under High Temperature Stress
In order to explore the changes in gene expression of strawberry seedling leaves under high tempera-ture stress after the addition of exogenous calcium ions,this experiment used'Hongyan'strawberry seedlings as m aterials and set up 4 experimental treatments,respectively as controls(day/night 25 ℃/17 ℃ 16 h/8 h)spray 20 mL of distilled water per plant,spray 20 mL of 8 mmol/L CaCl2 solution per plant,high temperature(day/night 42 ℃/34 ℃ 16 h/8 h)spray 20 mL distilled water per plant,spray 20 mL per plant at high temperature on the third day of high temperature stress,the leaves were taken for transcriptome sequencing and bioinformatics analysis.The results showed that,compared with the high temperature spraying distilled water treatment group,the high temperature spraying exogenous calcium ion treatment group had 2 452 genes were differentially expressed,of which 1 504 were up-regulated and 948 were down-regulated;GO enrichment analysis showed that in the cell composition,differen-tial genes were significantly enriched in cells,cell parts and organelles;the items that were significantly enriched in molecular functions were binding,catalytic activity,and structural molecular activity;significantly enriched bio-logical processes were metabolism process,cellular process,stimulus response,etc.KEGG enrichment analysis shows that the obvious enriched metabolic pathways include plant-pathogen interactions and MAPK plants signal transduction pathways-plant and plant hormone signal transduction,etc.Further analysis showed that exogenous calcium could make some genes encoding calcium sensing protein,WRKY transcription factor,MYB transcription factor,heat shock transcription factor and heat shock transcription protein express differently in response to high temperature stress,which provided valuable data information for exploring the role of Ca2+signal in the mecha-nism of strawberry seedlings in response to high temperature stress.
Strawberry seedlingsCaCl2High temperature stressTranscriptome
NETL
NSTL
万方数据
