摘要
为探究添加外源钙离子后高温胁迫下草莓幼苗叶片的基因表达变化情况,本试验以'红颜'草莓幼苗为材料,设置4个试验处理,分别为对照(昼/夜25 ℃/17 ℃ 16 h/8 h)每株喷施20 mL蒸馏水、对照每株喷施20 mL的8 mmol/L CaCl2溶液、高温(昼/夜42 ℃/34 ℃ 16 h/8 h)每株喷施20 mL蒸馏水、高温每株喷施20 mL的8 mmol/L CaCl2溶液,在高温胁迫第3天时取叶片进行转录组测序和生物信息学分析.结果表明:与高温喷施蒸馏水处理组相比,高温喷施外源钙离子处理组发生差异表达的基因共有2 452个,其中1 504个上调表达,948个下调表达;GO富集分析显示在细胞组成中,差异基因显著富集在细胞、细胞部分和细胞器;在分子功能中显著富集的条目是结合、催化活性、结构分子活性;显著富集的生物过程为代谢过程、细胞过程、刺激应答等;KEGG富集分析表明显著富集的代谢通路有植物与病原体的相互作用和MAPK植物信号传导途径-植物和植物激素信号转导等,进一步分析发现添加外源钙离子会使一些编码钙离子感受蛋白、WRKY转录因子、MYB转录因子、热激转录因子和热激转录蛋白的基因响应高温胁迫而发生差异表达,这为探究Ca2+信号在草莓幼苗应对高温胁迫机制中发挥的作用提供有价值的数据信息.
Abstract
In order to explore the changes in gene expression of strawberry seedling leaves under high tempera-ture stress after the addition of exogenous calcium ions,this experiment used'Hongyan'strawberry seedlings as m aterials and set up 4 experimental treatments,respectively as controls(day/night 25 ℃/17 ℃ 16 h/8 h)spray 20 mL of distilled water per plant,spray 20 mL of 8 mmol/L CaCl2 solution per plant,high temperature(day/night 42 ℃/34 ℃ 16 h/8 h)spray 20 mL distilled water per plant,spray 20 mL per plant at high temperature on the third day of high temperature stress,the leaves were taken for transcriptome sequencing and bioinformatics analysis.The results showed that,compared with the high temperature spraying distilled water treatment group,the high temperature spraying exogenous calcium ion treatment group had 2 452 genes were differentially expressed,of which 1 504 were up-regulated and 948 were down-regulated;GO enrichment analysis showed that in the cell composition,differen-tial genes were significantly enriched in cells,cell parts and organelles;the items that were significantly enriched in molecular functions were binding,catalytic activity,and structural molecular activity;significantly enriched bio-logical processes were metabolism process,cellular process,stimulus response,etc.KEGG enrichment analysis shows that the obvious enriched metabolic pathways include plant-pathogen interactions and MAPK plants signal transduction pathways-plant and plant hormone signal transduction,etc.Further analysis showed that exogenous calcium could make some genes encoding calcium sensing protein,WRKY transcription factor,MYB transcription factor,heat shock transcription factor and heat shock transcription protein express differently in response to high temperature stress,which provided valuable data information for exploring the role of Ca2+signal in the mecha-nism of strawberry seedlings in response to high temperature stress.
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