Identification and Expression Analysis of TPS Genes Family in Grape
In order to clarify the expression characteristics of TPS gene family in grape under abiotic stress,based on the analysis of biological information,the cuttings of Vitis vinifera'Yinhong'grape were taken as the experi-mental material.The effects of salt and light stress on the expression of TPS family genes in grape were examined by RT-qPCR.The results showed that there were 9 members of the TPS gene family,which could be divided into 3 subfamilies and distributed on 8 chromosomes.Multiple sequence alignments revealed that Vitis vinifera,Ara-bidopsis thaliana and Oryza sativa had a characteristic sequence(G-X-FLH-X-PFPS-X-E-X5-P-X-R and X-F-X-T-X-D-X-ARHF-X2).Motif analysis showed that VvTPS06 and VvTPS07 genes contained the same conserved amino acid sequence.Subcellular localization predicted that grape TPS genes were mainly distributed in cytoplasm,chloroplast and nucleus.VvTPS06,VvTPS07 and VvTPS09 were distributed in chloroplast,we speculated that they may be related to photosynthesis.The protein secondary structure showed that grape TPS protein mainly consisted of alpha helix and random coil.The microarray data showed that VvTPS02,VvTPS03,VvTPS05 and VvTPS07 all have a high expression in different periods and tissues,especially VvTPS07.The results of RT-qPCR analysis showed that the expression patterns of VvTPS genes were similar under salt stress,and the expression levels of VvTPS05,VvTPS07 and VvTPS09 were increased to a certain extent under high salt stress(0.6％).This showed that VvTPS05,VvTPS07 and VvTPS09 were bound up with plant salt tolerance,and it would provide a reference for the research on abiotic stresses to grape.In shading stress,when shading rate was 90％,the expression of VvTPS02,VvTPS07 and VvTPS09 were increased significantly compared with control group.It is speculated that the expression levels of VvTPS02,VvTPS07 and VvTPS09 were increased to promote photosynthesis and make up for the lack of light.
TrehaloseGrapeGene family of TPSAbiotic stressRT-qPCR
NETL
NSTL
万方数据
