基于重测序的'蒙冠1号'、'蒙冠2号'全基因组遗传变异分析
Genetic Variation Analysis of the Whole Genome of'Mengguanl'and'Mengguan2'Based on Resequencing
陆昕 1王晗慧 2曹丽 2李显玉 3乌志颜 3关岛 4杨素芝 3李英爽 5郑志民2
作者信息
- 1. 东北林业大学林学院,林木遗传育种国家重点实验室,哈尔滨,150040;赤峰市林业科学研究所,赤峰,024000;国家林业草原文冠果工程技术研究中心,赤峰,024000
- 2. 东北林业大学林学院,林木遗传育种国家重点实验室,哈尔滨,150040
- 3. 赤峰市林业科学研究所,赤峰,024000;国家林业草原文冠果工程技术研究中心,赤峰,024000
- 4. 赤峰市林业和草原局,赤峰,024000
- 5. 赤峰市森林草原保护发展中心,赤峰,024000
- 折叠
摘要
为了研究不同文冠果品种之间的遗传变异,采用高通量重测序技术,对两个高产文冠果品种'蒙冠1号''蒙冠2号'分别进行了全基因组测序,平均测序深度为93.35x和86.76x,覆盖率为99.96％,并与参考基因组比对,在两个品种中检测到了丰富的遗传变异,包括SNP和InDel.对存在遗传变异的基因进行GO富集,发现它们主要富集在细胞壁合成和胁迫响应.针对不同品种的差异,发现'蒙冠1号'主要富集在细胞壁生物发生及生长发育通路,'蒙冠2号'主要与胁迫响应和防御通路相关,推测这些基因的遗传变异是不同性状差异的原因.本研究结果一定程度上反映了文冠果两个品种之间在基因组水平的差异,为分子标记的开发提供了遗传基础,也为后续的分子育种及品种改良提供了理论指导和科学依据.
Abstract
In order to study the genetic variation among different variations of Xanthoceras sorbifolium Bunge species,the whole genomes of two high-yielding'Mengguanl'and'Mengguan2'were sequenced using high throu-ghput resequencing technology.The average sequencing depth was 93.35x and 86.76x,and the coverage rate was 99.96％.Compared with reference genome,rich genetic variations,including SNP and InDel,were detected in both cultivars.GO enrichment was performed on genes with genetic variation,and it was found that they were mainly enriched in cell wall synthesis and stress response.According to the differences among different varieties,'Meng-guanl'was mainly enriched in the cell wall biogenesis,growth and development pathways,while'Mengguan2'was mainly related to stress response and defense pathways.It was speculated that the genetic variation of these genes was the reason for different traits.To some extent,the results of this study reflect the differences between the two varieties at the genomic level,which provides the genetic basis for the development of molecular markers,and also provides theoretical guidance and scientific basis for the subsequent molecular breeding and variety improvement.
关键词
全基因组重测序/蒙冠1号/蒙冠2号/遗传变异/胁迫响应Key words
Whole genome resequencing/Mengguan1/Mengguan2/Genetic variation/Stress response引用本文复制引用
基金项目
内蒙古自治区"草原英才"青年创新人才工程专项()
内蒙古自治区林业科技支撑项目(内林科研[2021]12号)
内蒙古自治区科技重大专项(ZDZX2018056)
内蒙古自治区科技成果转化引导项目(2020CG0077)
出版年
2024
NETL
NSTL
万方数据