Genetic Variation Analysis of the Whole Genome of'Mengguanl'and'Mengguan2'Based on Resequencing
In order to study the genetic variation among different variations of Xanthoceras sorbifolium Bunge species,the whole genomes of two high-yielding'Mengguanl'and'Mengguan2'were sequenced using high throu-ghput resequencing technology.The average sequencing depth was 93.35x and 86.76x,and the coverage rate was 99.96％.Compared with reference genome,rich genetic variations,including SNP and InDel,were detected in both cultivars.GO enrichment was performed on genes with genetic variation,and it was found that they were mainly enriched in cell wall synthesis and stress response.According to the differences among different varieties,'Meng-guanl'was mainly enriched in the cell wall biogenesis,growth and development pathways,while'Mengguan2'was mainly related to stress response and defense pathways.It was speculated that the genetic variation of these genes was the reason for different traits.To some extent,the results of this study reflect the differences between the two varieties at the genomic level,which provides the genetic basis for the development of molecular markers,and also provides theoretical guidance and scientific basis for the subsequent molecular breeding and variety improvement.
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