为揭示三角梅的遗传背景,本研究采用软件MicroSAtellite搜索其苞片转录组SSR位点,使用Primer Premier 5软件设计50对SSR引物,将筛选出的多态性引物用于55份三角梅材料的聚丙烯酰胺凝胶电泳,采用Popgene 32、PowerMarkerV3.25和Structure V2.3.4等软件分析三角梅遗传多样性.结果显示,从50对SSR引物中筛选出25对条带清晰且多态性好的引物,25对SSR引物共检测到76个等位基因,平均每对引物有3个等位基因,平均有效等位基因数为2.30,引物多态性信息量(PIC)在0.01～0.66之间,均值为0.38.55个三角梅材料间的遗传距离为0.01～0.92,平均遗传距离为0.43;在遗传距离0.44处,聚类分析将55份材料分为3个群体.与印度三角梅栽培种比较而言,本实验研究的三角梅材料的遗传多样性水平不高,结果可为三角梅品种鉴定、保护及进化提供一定参考价值.
Analysis of Bougainvillea glabra Choisy Genetic Diversity Based on SSR of Transcriptome
To reveal the genetic background of Bougainvillea glabra.Transcriptome SSR sites of bracts in B.glabra were searched by software MicroSAtellite,fifty pairs of SSR primers were designed by Primer Premier 5.The selected polymorphic primers were applied to 55 B.glabra materials by polyacrylamide gel electrophoresis.Genetic diversity of B.glabra were analyzed by software Popgene 32,PowerMarker V3.25 and Structure V2.3.4.The results showed that 25 pairs of primers with clear bands and good polymorphism were selected from 50 pairs of SSR primers by research.And 76 alleles were detected in 25 pairs of SSR primers.Average per pairs of primers had three allelic genes and the average number of effective allelic genes was 2.30.Primers polymorphism informa-tion(PIC)ranged from 0.01 to 0.66,with a mean of 0.38.The genetic distance of 55 B.glabra ranged between 0.01 and 0.92 with an average of 0.43.Fifty-five materials were divided into 3 groups at the genetic distance of 0.44 by cluster analysis.The genetic diversity level of B.glabra in this experiment was not high comparing with the culti-vated species in India.The results can provide a certain reference value on variety identification,protection and evolution for B.glabra varieties.
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