Genetic Analysis of 33 Mango Varieties Based on Genotyping by Sequencing
Mango has abundant germplasm resources,and there are few studies on the correlation between mango breeding materials and disease resistance genetic structure and genetic relationship.In order to analyze and explore the genetic structure and genetic relationship of different mango varieties resistant and susceptible to bacterial angular spot disease,this study used GBS(Genotyping by sequencing)simplified genome sequencing technology to sequence 33 mango materials based on the SNP information after data processing.Phylogenetic tree analysis,principal component analysis and population genetic structure analysis,analysis of genetic evolution and genetic relationship of mango germplasm materials,combined with the results of early disease resistance evaluation experiments to explore its genetic differentiation characteristics and the correlation with disease resistance.The results shows that 33 mango varieties can be divided into 7 groups,group Ⅰ contains 5 of the 7 high and medium resistant varieties;group Ⅲ and group Ⅳ are close in genetic distance,including 3 of the 4 highly susceptible varieties;The optimal number of clusters for population structure analysis is 9;principal component analysis shows that there is no obvious grouping among these mango varieties,but the genetic distance and phylogenetic t ree results are basically the same.In the genetic diversity analysis,the expected heterozygosity(He)of all mango varieties is 0.336 1,the polymorphic information content(PIC)is 0.269 4,the effective numbers of alleles(Ne)is 1.573 2,and the Shannon information index(Ⅰ)is 0.504 1,indicating that the genetic diversity of mangoes is low.In addition,the genetic diversity indexes from high-resistant varieties to susceptible varieties were moderately susceptible＞susceptible＞moderately resistant＞high susceptible＞high resistant,indicating that the high susceptibility and high resistance of these varieties have higher genetic consistency than other susceptible varieties,while the genetic diversity of the moderately susceptible varieties is high.The susceptible cultivars have higher genetic con-sistency,while the moderately susceptible varieties have higher genetic diversity.The above results indicate that the genetic difference between the highly susceptible varieties and the highly resistant varieties is large and the genetic relationship is far;the population pedigree mixing may help to improve the disease resistance of the varieties to a certain extent.The research results provide theoretical basis for future mango hybrid breeding,germplasm creation,and development and utilization of mango germplasm resources.
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