Gene Cloning,Over-expression Vector Construction and Expression Analy-sis of FvSPL2 Gene in Fragaria vesca
SPL(SQUAMOSA promoter-binding protein-like)transcription factors are plant-specific gene family that are widely found in higher plants and play an important regulatory role in plant vegetative growth and repro-ductive development.This study investigates the effect of SPL genes on flower development in woodland strawberry.In this experiment,the SPL transcription factor gene FvSPL2 was cloned from woodland strawberry(Fragaria vesca)'Hawaii4',and the 35S::FvSPL2 over-expression vector was constructed based on the analysis of woodland straw-berry transcriptome data.The results showed that the coding region of FvSPL2 gene was 639 bp,encoding 212 amino acids.The physicochemical and structural analyses indicated that FvSPL2 protein was an unstable hy-drophobic protein,which contained a highly conserved SBP structural domain and a nuclear localization signal NLS.The highest expression of FvSPL2 gene was detected by RT-qPCR in woodland strawberry flowers,and the lower expression in petiole and leaf.Moreover,the expression level of FvSPL2 at different flowering stages showed an upward trend first and then downward,through period of flower buds(the lowest level),half-blooming flowers(the highest level)and full-blooming flowers,and the difference between the half flowering stage and other two periods was significant.These results indicated that this gene is mainly involved in the flowering regulation pro-cess.The results of the study provide data for the subsequent study of the biological functions of FvSPL2 gene in woodland strawberry.
NETL
NSTL
万方数据
