Effects of Different Chemical Treatments on Surface Bacterial Community and Germination of Black Kidney Bean Seeds
Seed sterilization is an essential measure to prevent seedling diseases and cultivate healthy seedlings.In this study,the diversity and structural characteristics of surface bacterial communities and the abundance change of potential pathogens of black kidney bean seeds after treated by three different agents were detected using Illumina MiSeq sequencing technology.And the germination indexes including seed germination rate,germination poten-tial,germination index,radicle,hypocotyl,dry weight and fresh weight were measured.It will provide data support for scientific selection of disinfectants for black kidney bean seeds.The results showed that:21 phyla,41 classes,65 orders,128 families and 215 genera were detected in the surface bacterial community.Proteobacteria(90.59％)were the first dominant phyla,followed by Actinobacteria(7.22％)and Bacteroidetes(0.73％).There were differences in the diversity and richness of bacterial communities among three chemical treatments.The relative abundance of Xanthomonas,Curtobacterium,Pantoea,Ralstonia and Pectobacterium decreased significantly.3％Zhongshengmycin was the best seed treatment,the abundance of Pectobacterium,Ralstonia and Xanthomonas decreased significantly by 100％,0.79％and 3.05％respectively.The abundance of Curtobacterium and Pantoea decreased by 0.98％and 0.76％respectively.The germination index of black kidney bean seeds and growth index of seedlings were signifi-cantly higher than those of CK after chemical treatment,and there were differences among different chemical treat-ments.Compared with the control,the germination rate and germination potential increased by 11.94％and 16.55％,the radicle and hypocotyl elongated by 3.50 cm and 2.48 cm.The dry and fresh weight improved by 1.58 g and 2.79 g respectively,which were significantly different from the control(P＜0.05).
Black kidney beansChemical treatmentHigh throughput sequencing technologyDiversity of sur-face bacteria communitiesSeed germination indexes
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NSTL
万方数据
