Preliminary Establishment of Tissue Culture and Rapid Propagation System of'Liaohong'A ilanthus altissima
The new variety of'Liaohong'Ailanthus altissima(Certificate No.20190262)was bred from a bud strain of Ailanthus altissima by us.The establishment of the tissue culture and rapid clonal propagation system of'Liaohong'Ailanthus altissima is not only beneficial to popularize the variety rapidly,but also helpfull the study of the formation mechanism of pod color.The effects of disinfection methods,different hormone ratio on the culture medium MS of callus formation,subculture,adventitious bud formation and rooting of'Liaohong'Ailanthus altissima were studied,through selecting axillary buds and top buds as explants.The results showed that the disinfection methods of axillary buds and top buds were soaked in 75％alcohol for 30 s,15％sodium hypochlorite solution for 15 minutes,and then 0.1％mercuric chloride solution for 6 minutes,the tissue pollution rate was 20.00％,and the success rate of callus induction was 40.00％;the ratio of hormone in MS medium for dedifferen-tiation induction and subculture was 0.1 mg/L 6-BA,0.1 mg/L NAA+2,4-D,and the induction rate of callus reached 40.00％,and the callus grow better;the optimal ratio of hormone in MS for adventitious bud formation was 3.0 mg/L 6-BA,0.05 mg/L NAA+2,4-D,and the induction rate of adventitious buds reached 82.33％;the optimal medium MS for rooting was NAA 2.0 mg/L and the induction rate of adventitious root was 58.33％.The tissue culture of'Liaohong'A ilanthus altissima can meet the needs of rapid propagation of'Liaohong'A ilanthus altissima.It has the advantages of easy production,low cost,easy operation and large propagation coefficient.The results of this study can provide some technical support for industrial nursery of'Liaohong'A ilanthus altissim.
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