Bioinformatics Analysis of AcNOD Gene from Actinidia chinensis
Nodulin(NOD)is a kind of plant gene product that mainly exists in nodule cells and participates in sym-biotic nitrogen fixation,and is found in other tissues and cells of leguminous plants and non-leguminous plants.To explore the biological function of the NOD gene in non-Leguminosae plants such as Actinidia chinensis,this study used a series of bioinformatics methods to analyze its sequence characteristics.The results showed that the number of amino acid residues of the protein encoded by the gene is 94,the molecular formula of the protein is C432 H715N131O120S3,the isoelectric point pI of the protein is 10.68,which belongs to hydrophobic protein.There are 11 positively charged residues(Arg+Lys)and 3 negatively charged residues(Asp+Glu),the instability coefficient is 14.00,the fat coefficient is 107.23,and the total average hydrophilic coefficient is 0.395;AcNOD protein con-tains a conserved domain of ENOD93;the results of the secondary structure prediction showed that the protein is mainly composed of 59.57％α-helix,17.02％random curl,15.96％extended chain and 7.45％β-turn;the results of the tertiary structure prediction showed that the protein is twisted and folded more,subcellular localization is in the endoplasmic reticulum,there are 12 phosphorylation sites of 4 glycosylation sites,no signal peptide se-quence,and two transmembrane regions.The results of phylogenetic tree analysis showed that the protein has high homology with leguminous nodulin,and has a similar or identical function.The results provided basic data for further understanding the mechanism of the plant NOD gene and the biological function of the A cNOD gene in Actinidia chinensis.
万方数据
维普
