Cloning and Expression Analysis of Tobacco SnRK2 Gene Family
Crops suffer a variety of abiotic stresses during their growth and development,which have great effects on the yield and quality.There are various abiotic stresses and drought is one of the main stresses.Tobacco is an important crop,mainly grown in the southwestern regions of China,where plants meet frequent drought stress.The SnRK2 gene family is involved in the process of drought resistance in plants and can be induced by stress,which is an important manifestation of the function of the SnRK2 gene family.In order to study the potential functions of SnRK2 genes in tobacco,the Group I genes of tobacco SnRK2 gene family were first cloned.The expression pat-terns of tobacco SnRK2 genes were detected in different tissues and under stresses.The results showed that the ex-pression of Ntab0922420 gene in tobacco SnRK2 gene was the highest,and the members of tobacco SnRK2 gene family were more easily expressed in stems and leaves.Following exposure to low temperature,salt,drought stress,and ABA treatment,some members of the SnRK2 genes exhibited stress-induced expression,among which the gene with the ID Ntab0894060 was significantly induced by drought.These efforts will lay the groundwork for the functional identification of the tobacco SnRK2 gene family and provide genetic resources for breeding to-bacco varieties resistant to adversity.