首页|烟草SnRK2基因家族的克隆及表达分析

烟草SnRK2基因家族的克隆及表达分析

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农作物生产过程中会面临多种非生物胁迫的影响,会极大地影响作物的产量以及品质.非生物胁迫形式多种多样,干旱胁迫是其中一种主要形式.烟草是重要的经济作物,主要在中国西南地区种植,但经常会遭受极端干旱胁迫.SnRK2基因家族参与植物抗旱过程,能够受逆境胁迫诱导表达,这种表达是SnRK2基因家族功能的重要体现.为了进一步研究烟草SnRK2家族基因的功能,本研究克隆了烟草SnRK2基因家族Group I的基因,并检测了烟草SnRK2基因家族的组织表达特异性及诱导表达特异性.结果表明,烟草SnRK2基因中Ntab0922420基因的表达量最高,同时烟草SnRK2基因家族成员更容易在茎与叶中表达.烟草经过低温、盐、干旱胁迫以及ABA处理后,部分SnRK2基因成员表现出受胁迫诱导,其中ID为Ntab0894060的基因受干旱显著诱导.这些工作将为研究烟草SnRK2基因家族功能鉴定基础,并为培育抗逆烟草品种提供遗传资源.
Cloning and Expression Analysis of Tobacco SnRK2 Gene Family
Crops suffer a variety of abiotic stresses during their growth and development,which have great effects on the yield and quality.There are various abiotic stresses and drought is one of the main stresses.Tobacco is an important crop,mainly grown in the southwestern regions of China,where plants meet frequent drought stress.The SnRK2 gene family is involved in the process of drought resistance in plants and can be induced by stress,which is an important manifestation of the function of the SnRK2 gene family.In order to study the potential functions of SnRK2 genes in tobacco,the Group I genes of tobacco SnRK2 gene family were first cloned.The expression pat-terns of tobacco SnRK2 genes were detected in different tissues and under stresses.The results showed that the ex-pression of Ntab0922420 gene in tobacco SnRK2 gene was the highest,and the members of tobacco SnRK2 gene family were more easily expressed in stems and leaves.Following exposure to low temperature,salt,drought stress,and ABA treatment,some members of the SnRK2 genes exhibited stress-induced expression,among which the gene with the ID Ntab0894060 was significantly induced by drought.These efforts will lay the groundwork for the functional identification of the tobacco SnRK2 gene family and provide genetic resources for breeding to-bacco varieties resistant to adversity.

TobaccoSnRK2Expression analysis

白戈、杨大海、费明亮、谢贺

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云南省烟草农业科学研究院,烟草生物技术重点实验室,国家烟草基因工程研究中心,昆明,650021

烟草 SnRK2 表达分析

中国烟草总公司云南省公司科技计划项目中国烟草总公司科技计划项目

2021530000241016110202001018

2024

分子植物育种
海南省生物工程协会

分子植物育种

CSTPCD北大核心
影响因子:0.765
ISSN:1672-416X
年,卷(期):2024.22(12)
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