Analysis of Leaf Color Related Genes Based on Transcriptome Sequencing in Prunus cerasifera Ehrhar f.atropurpurea Rehd.
Prunus cerasifera Ehrhar f.atropurpurea Rehd.is a kind of deciduous tree with high ornamental value.Leaf color is one of the important ornamental characters of P.cerasifera.In order to explore the key enzyme genes affecting anthocyanin synthesis of P.cerasifera,in this study,transcriptome sequencing of P.cerasifera with green leaf and red leaf phenotype was performed using Illumina NovaSeq6000 sequencing platform.In this experiment,195 548 106 clean reads were obtained with red P.cerasifera as the experimental group,green leaf and common leaf as the control group,and each sample had 3 biological replicates.5 376 differentially expressed genes in red and green leaves were screened,including 2 686 up-regulated genes and 2 690 down-regulated genes.There were 6 197 differentially expressed genes in red leaves,including 3 193 up-regulated genes and 3 004 down-regulated genes.According to GO functional classification,it can be divided into three categories:biological process,cell component and molecular function.Among them,red leaf of P.cerasifera and green leaf of P.cerasifera were compared to 44 functional groups,and red leaf of P.cerasifera and common leaf of P.cerasifera were compared to 47 functional groups.KEGG pathway analysis showed that there were 897 differentially expressed genes involved in 120 known metabolic pathways in the ratio of red leaf of P.cerasifera to green leaf of P.cerasifera,and 1 108 differentially expressed genes involved in 121 known metabolic pathways in the ratio of red leaf of P.cerasifera to common leaf of P.cerasifera.There were significant differences in the expression of some genes in the three anthocyanin-related metabolic pathways:phenylpropanin synthesis pathway,flavonoid synthesis pathway,and flavonoid and flavonol synthesis pathway.The expression patterns of anthocyanin synthesis-related differentially expressed genes in three different leaf of P.cerasifera were verified by real-time fluorescence quantitative analysis.This study enriched the transcriptome information of P.cerasifera,and provided a scientific basis for further study on the molecular mechanisms related to leaf coloring and the cloning and expression of growth-related genes.
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