Cloning and Expression Pattern Analysis of CiS40-11 Gene from Caragana intermedia
CiS40-11 is a member of SnR family from Caragana intermedia.In order to understand its function in abiotic stress,the ORF of CiS40-11 was cloned,and its tissue specificity and abiotic stress expression pattern was analyzed.The ORF of CiS40-11 is 633 bp,CiS40-11 composed of 210 amino acids,its molecular weight is about 23.06 kD and the isoelectric point is 5.70.Tissue-specific analysis shows that the maximum expression of CiS40-11 is showed in leaves.In addition,the expression of CiS40-11 continuously decreased under cold and drought stress treatment.This study provides a theoretical basis for further functional analysis and regulation mechanism of CiS40-11 in the process of abiotic stress.
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维普
