In order to ascertain the composition of functional markers for quality traits in 110 varieties(lines),we detected these materials with high-throughput KASP markers.These quality traits included 1B/1R translocation line,high molecular weight glutenin subunits(HMW-GS),low molecular weight glutenin subunits(LMW-GS),kernel hardness,flour color,seed color.The results showed the tested materials contained 1B/1R translocation lines with the proportion of 43.64％.HMW-GS genes Glu-Ax1 and Glu-Ax2*accounted for 50.91％and 3.64％,re-spectively,and the proportion of 1Dx5+1Dy10 at Glu-Dl loci was 16.36％.The proportion of the favorable allele for kernel hardness detected by Pina-D1b and Pinb-D1b were 4.55％and 95.46％,respectively,and that of PPO-D1 was small,only accounted for 4.55％.For flour color,the proportion of Psy-B1a or Psy-B1b,TaLyc-B1b and Psy-A1b were 95.45％,77.27％and 50％,respectively.Tamby-D1b,the main effector gene controlling seed color,was not detected in the test material,while Tamby-A1b accounted for 5.45％,and the glutinous gene Wx-B1b ac-counted for a smaller proportion of 4.54％.These functional markers had various proportions in different breeding department.In conclusion,this study identified the alleles of quality trait related genes using KASP markers,pro-vided reference for further marker-assisted selection breeding of wheat quality traits in the region.
维普
万方数据
