Isolation and Identification of the Pathogen of Camellia oleifera Anthracnose
Camellia oleifera Abel.is a plant of Theaceae and Camellia L.,which is known for its high oil content and significant economic value.However,there are a variety of fungal diseases in Camellia oleifera,especially the anthrax of Camellia oleifera,which poses a serious threat to China's Camellia oleifera industry.In order to delve deeper into the disease,in this study,pathogen A and pathogen B were isolated from Camellia oleifera leaves using conventional tissue isolation.They were cultured on PDA for 5 d with preliminary identification through detailed morphological observation;perform ITS sequence analysis on pathogen A and pathogen B,and perform homology alignment in GenBank,to further clarify its classification status.Pathogenicity testing is conducted through in vitro and in vivo inoculation.The results showed that combined with morphological and molecular identification results,pathogen A was a weak pathogen and identified as Diporthe lichocarpus;pathogen B was a strong pathogenic bac-terium identified as Colletotrichum camelliae.These findings not only contribute to a better understanding of the pathogenic mechanism of Camellia anthracnose but also provide a crucial foundation for the development of tar-geted prevention and control strategies.
万方数据
维普
