Resynthesis of Yellow-seeded Brassica nap us and Comparative Metabonomic Analysis of Differently Colored Seed Coats
To study the stability of resynthesized Brassica napus and the differences of accumulated metabolites be-tween the yellow and black seed coats of resynthesized B.napus,the cytological stability of an A4 line of resynthe-sized B.napus was investigated.Then vanillin staining and widely targeted metabolomics were conducted in seed coats of yellow and black seeded A10 lines which derived from self-pollination of the A4 line.The results showed that the A4 line exhibited normal mitotic and meiotic behaviors with a somatic chromosome number of 38.The color dif-ference of the seed coats in these two A10 lines related with the accumulation of proanthocyanidins.The accumula-tion of proanthocyanidins in black-seeded coats were increased gradually after 30 days after flowering(DAF),whereas little proanthocyanidins were detected in yellow-seeded coats.A total of 737 metabolites from 23 classes were detected in the yellow and black seed coats at 50 DAF.A sum of 343 differentially accumulated metabolites were identified.Compared with black seed coats,149 and 194 metabolites accumulated significantly higher and lower in yellow seed coats,respectively.The differentially accumulated metabolites are mainly enriched in three metabolic pathways,including flavone and flavonol biosynthesis,glycerolipid metabolism and anthocyanin biosyn-thesis.In summary,the different seed coat color in this resynthesized B.napus attributes to the different accumula-tion of proanthocyanidins and other flavonoid metabolites in the seed coats.The results laid a theoretical foundation for revealing the mechanism of seed coat color formation,and provided new germplasm for breeding yellow-seeded B.napus varieties.
Resynthesized B.napusYellow seedMetabolomicsProanthocyanidinsSeed coat color
万方数据
维普
