Secondary Metabolites and Antibacterial Activity of Endophytic Fungus Fusarium falciforme R16 from the Patchouli
Patchouli(Pogostemon cablin)is a famous medicinal plant from southern China and is of wide applica-tion value.In order to explore the metabolic properties and antibacterial effects of endophytic fungus Fusarium fal-ciforme R16,tissue cul ture was used to isolate and identify endophytic fungi from flower tissues of P.cablin.Com-pounds were isolated from the extract of fermentation of this fungus by chromatography column on silica gel and semi-preparative reversed phase HPLC,and their structures were elucidated by NMR and mass spectrometry analy-ses.All the compounds were evaluated for their bacteriostat activity.As a result,twelve compounds were isolated and identified,including p-hydroxyphenethylalcohol(1),2-(4-biphenylyl)ethanol(2),4-methyl-5,6-dihy dropyren-2-one(3),ethyl phenylacetate(4),anisole(5),4-bromostilbene-O-methyl isopropionate(6),2-met-hyl-1,4-benzenediol(7),N-(4-hydroxyphenethyl)-2-phenylacetamide(8),4-pentenyl acetate(9),2-(4-met-hoxyphenyl)propan-2-ol(10),4-methylideneoxane(11)and 2-tetralnaphthol(12).Compounds 5,7 and 10 showed definite inhibitory activities against Staphylococcus aureus and Bacillus subtilis and compounds 11 and 12 showed weak inhibitory activities against S.aureus,the theory basis for discovery of antibacterial active substances was provided.
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