Screening and Verification of Transcription Factor IbMYB1-Interacting Proteins in Purple Sweet Potato
IbMYB1 is an important transcription factor that regulates anthocyanin biosynthesis in storage roots of purple sweet potato.In order to identify new proteins that interact with transcription factor IbMYB1 and participate in anthocyanin biosynthesis and regulation,in this study,we screened the interacting proteins through yeast two-hybrid library,and obtained the gene sequences of 91 potential interacting proteins.According to the GO annotation and statistical analysis of the selected genes,5 candidate genes that may be related to anthocyanin biosynthesis were preliminarily determined,namely IbCPK,IbMPK3,IbCSN3,IbFbox-5 and IbMPK4.The in-teraction between the candidate protein and IbMYB1 was re-verified by the yeast two-hybrid rotation experiment and the bimolecular fluorescence complementation experiment.It was finally determined that IbCSN3 and IbMPK4 were interacting proteins that interact with IbMYB1 and may participate in the regulation of anthocyanin bio-synthesis.This study provides an experimental reference for the identification of IbMYB1 interaction proteins,and provides new clues for enriching the molecular network of anthocyanin biosynthesis and regulation in purple sweet potato.
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