Analysis of Biological Information and Expression Pattern of CmbZIPs Gene in Ground-cover Chrysanthemum
Ground-cover chrysanthemum(Chrysanthemum morifolium)is a perennial herbaceous flower of Dendran-thema genus in the family Compositae,in which has the characteristics of dense flowers,rich flower colors and strong stress resistance,and is an excellent variety for landscaping.However,the distribution of ground cover-chrysan-themum is restricted by low temperature and soil salinization.The HD-ZIP family is widely involved in abiotic stress in plants.In this study,CmbZIP12,CmbZIP24,CmbZIP10,CmbZIP3 genes were cloned from ground-cover chrysan-themum based on transcriptome data,and its amino acid sequence and conserved domain were analyzed by bioinfor-matics.Under low temperature and NaCl abiotic stress,the expression characteristics of these genes were analyzed by RT-qPCR.The results showed that the ORF lengths of CmbZIP12,CmbZIP24,CmbZIPI0,and CmbZIP3 genes were ranged from 453 to 630 bp.CmbZIP12 and CmbZIP3 were acidic proteins,and CmbZIP24 and CmbZIP10 were basic proteins;CmbZIP12,CmbZIP10 and CmbZIP3 were unstable proteins,and CmbZIP24 was a stable protein.Four conserved motifs were identified by MEME software.Phylogenetic analysis showed that CmbZIP12,CmbZIP3 belonged to HD-ZIPⅠ subfamily;CmbZIP10 and CmbZIP24 belong to HD-ZIPⅡ and HD-ZIPⅢ sub-family,respectively.Interaction network prediction showed that CmbZIP12 had network interaction with GBF3,SNRK2.3,OST1 and other proteins;CmbZIP24 interacted with AT5G04840,ZIP2,BZIP34 and other proteins.The relative expression levels of CmbZIP12,CmbZIP24,CmbZIP10 and CmbZIP3 were significantly increased by low temperature and salt treatment.This study can provide reference for the functions of genes CmbZIP12,Cmb-ZIP24,CmbZIP10 and CmbZIP3 in ground-cover chrysanthemum.
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