Metabolomics Analysis of Astragalus mongolicus Seed Aging
Astragalus mongholicus Bunge is a perennial herb,one of the source plants of traditional Chinese medicine Radix Astragali.Its seeds lose vigor quickly under conventional storage conditions,which is not benefi-cial to the development of Astragalus industry.In order to explore the changes of metabolites in the aging process of Astragalus mongolicus seeds and reveal the aging mechanism of seeds,this study used artificial aging method to obtain four kinds of seeds with different vigor levels,and then used non-targeted metabolomics technology to de-tect the different metabolites of Astragalus mongolicus seeds with different vigor levels.A total of 10 346 metabo-lites were detected,including 5 946 positive ions and 4 400 negative ions.Including alkaloids and their derivatives,benzoates,lignin and their compounds,lipids and lipid molecules,nucleosides and nucleotide analogues,organic acids and their derivatives,organic nitrogen compounds,organic oxygen compounds,organic heterocyclic com-pounds,organic sulfur compounds,phenylpropanes and polyketones compounds,unclassified substances,etc.Five metabolites(5,10-methylene-THF,dihydrofolic acid,stachyose,raffinose,GlcCer(d18:1/24:1(15Z)),which were closely related to the aging of Astragalus mongolicus seeds,were screened out by KEGG database analysis.Three metabolic pathways(Folate-mediated one-carbon metabolism,galactose metabolism and sphingolipid metabolism)were involved.These metabolites and metabolic pathways could provide reference for the study of seed aging mechanism.
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