Identification,Bioinformatics and Expression Pattern Analysis of the Tify Gene Family in Alfalfa(Medicago sativa L.)
Tify gene family is specific gene family in the plant,which plays an important role in plant growth and development,response to stresses and signal transduction.In order to study the response of Tify gene in alfalfa under abiotic stress,84 Tify genes were identified in alfalfa,and their chromosome location and gene structure were analyzed by bioinformatics.MsTify6,MsTify32,MsTify46,MsTify48 and MsTify65 are randomly selected for RT-qPCR analysis under salt,alkali and drought stresses.Physical and chemical properties and structural analysis reveal that,apart from MsTify1,MsTify2,and MsTify3,the remaining 81 genes are unevenly distributed across seven chromosomes,excluding chromosome 7.RT-qPCR result showed that MsTify gene had obvious response to salt,alkali,drought and other abiotic stresses,and played a key role in the regulation of adversity stress.It provides a new theoretical basis for the follow-up study of Tify gene.
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