Major QTL Analysis of Fruit Shape Related Traits in Bottle Gourd Based on BSA Method
Fruit shape is an important agronomic and commercial trait of bottle gourd.To locate the major genes controlling fruit shape-related traits in bottle gourd,this study used the bottle gourd cultivars'JH-8'with gourd shape,'Changhu21'with long club shape,and their F2 population(324 individual plants)as experimental materi-als.Extreme individual plants were selected to establish a gourd shape mixed pool and a long club shape mixed pool.Combined with high-throughput sequencing,QTLs related to fruit shape traits were screened.Using the SNP-index algorithm,three main loci associated with the regulation of fruit shape in bottle gourd were identified,located on Chr.3:6 765 000～9 431 999 kb,Chr.8:9 066 000～9 172 999 kb and Chr.9:14 000～2 908 999 kb,re-spectively.The results of this study provided an important candidate region for mapping bottle gourd fruit shape related traits,and are helpful for the fine mapping and cloning of target genes in the future.
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万方数据
