Selection and Validation of Reference Genes for qPCR in'Sally'Across Stages and Tissues
In order to select the internal reference genes stably expressed in different developmental stages of longiflorum hybird bulbs and different tissues at the same developmental stage,this study took longiflorum hybird cultivar'Sally'as the research object,and used quantitative real-time PCR(qPCR)to analyze the 6 levels of lily.The amplification efficiency of two candidate internal reference genes(ACT,BHLH,EF1,EIF,GAPDH,lilyact)in different developmental stages of'Sally'scales and in different tissues,then use Normfinder,Bestkeeper,geNorm,comparative ΔCt method and RefFinder softwares or methods to evaluate the stability of the expression of candi-date internal reference genes.The results showed that EIF and BHLH have the best comprehensive stability in all samples and in bulbs at different developmental stages.And in different tissues,GAPDH and EIF are most stable.In all samples and different tissues,ACT has the worst stability.The results of this study provide a suitable combi-nation of internal reference genes for the analysis of the expression levels of different developmental stages and different tissues of'Sally'bulbs,which lays the foundation for the subsequent study of gene expression levels.
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