Effects of Genomic DNA Extraction on Microbial Pretreatment from Areca Leaf
In order to rapidly obtain high-quality genomic DNA from areca leaf surfaces,this research utilized orbital shaking and ultrasonication to dislodge the microbes,followed by enrichment through vacuum filtration and high-speed centrifugation at low temperatures.The concentration and purity of the extracted microbial DNA were measured using a micro volume spectrophotometer.Finally,the effectiveness of the DNA extraction was compared through DNA electrophoresis and PCR amplification tests.When the ultrasonic method was used as a pretreatment,the concentration of genomic DNA was low and the purity was not within the acceptable range.The DNA concentration extracted by the orbital shaking method was higher,and reached its maximum values[(25.67±0.96)μg/mL and(38.67±0.85)μg/mL]after 20 minutes of shaking.Compared to the centrifugal method,the vacuum filtration method achieved higher DNA concentration and purity.The best method for collecting microorganisms from the surface of areca leaves was a combination of orbital shaking and vacuum filtration,which improved the efficiency and quality of microbial collection and also increased the success rate of DNA extraction from leaf surface microorganisms.The results of this study can provide a reference for the collection of microorganisms on the surface of areca leaves.
Areca catechu L.Microorganism on leaf surfacePretreatmentMicrobial collectionDNA extraction
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