首页|基于胚性愈伤诱变的楸树耐盐种质创制及评价

基于胚性愈伤诱变的楸树耐盐种质创制及评价

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楸树(Catalpa bungei)是中国特有的落叶硬木类珍贵树种,具有速生、耐盐碱、材质优良等优点,是沿海滩涂和内陆盐碱地土壤修复和防护的优良树种.创制新的耐盐种质,培育耐盐新品种,提高楸树耐盐能力,是目前急需解决的科学问题.本研究以楸树品系'钟山5号'胚性愈伤为材料,利用137Cs-γ射线结合耐盐定向筛选创制新的耐盐种质.结果表明,随着辐射剂量、盐浓度的逐渐增加,胚性愈伤的褐化率均逐渐增加,增殖率降低,因此,定向诱变最适的辐射剂量为40 Gy,最适的盐浓度为300 mmol/L NaCl.将用40 Gy处理后的愈伤放置在含300 mmol/L NaCl的再生培养基中,获得65株楸树再生幼苗,又经过苗期二级耐盐性评价获得3份耐盐的楸树新种质.本研究不仅为楸树耐盐品种培育提供新的种质材料,也为楸树耐盐基因克隆及机理研究提供科学依据.
Salt-tolerant Germplasm Creation and Evaluation of Catalpa bungei Based on Embryogenic Callus Mutagenesis
Catalpa bungei is a deciduous hardwood tree native to China with the advantages of fast-growing,salt resistance and excellent wood qualities.It is an excellent tree species for soil restoration and protection in coastal beach and saline land.Therefore,in order to improve the salt tolerance of C.bungei,it is the key scientific issue to create novel salt-tolerant germplasms and breeding new salt-tolerant varieties.In the present study,we created nov-el salt-tolerant germplasms using 137Cs-γ rays mutagenesis and targeted screening technologies of embryogenic callus of C.bungei'Zhongshan5'.Results showed that with the increases of radiation dose and salt concentration,the browning rate of embryogenic callus gradually increased,while the proliferation rate decreased.Therefore,the threshold of radiation dose for mutagenesis was 40 Gy,and the threshold of salt tolerance screening was 300 mmol/L NaCl.Subsequently,we radiated the embryogenic callus of'Zhongshan5'using 137Cs-γ rays at 40 Gy,and then transferred them to regenerating medium containing 300 mmol/L NaCl.After differentiation and regeneration of embryogenic callus,65 individuals were produced.Three new salt-tolerant lines were selected through sec-ondary salt tolerant evaluation at the seedling stage.This study would not only provide new germplasms for devel-oping new salt-tolerant varieties,but also lay a foundation for the salt-tolerant mechanism analysis and related genes cloning in C.bungei.

Embryogenic calli137Cs-γ raysGermplasms creationSalt-tolerance evaluation

高露璐、王鹏、顾姣姣、姜聖姬、李亚、赵明杨、吕芬妮

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江苏省中国科学院植物研究所,南京,210014

睢宁县自然资源和规划局,徐州,221200

胚性愈伤 137Cs-γ射线 种质创制 耐盐评价

中央财政林业科技推广示范资金项目国家自然科学基金面上项目江苏省植物资源研究与利用重点实验室开放基金江苏省中国科学院植物研究所博士科研人员启动资金项目江苏省林业三新工程项目

苏[2018]TG0132071794JSPKLB201929JSPKLB202021LYKJ[2017]05

2024

10.13271/j.mpb.022.004357

分子植物育种
海南省生物工程协会

分子植物育种

CSTPCD北大核心
影响因子：0.765
ISSN：1672-416X
年,卷(期)：2024.22(13)
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