Cloning of BnaJAZ1 Gene Related to Salt Tolerance in Brassica napus and Analysis of BnaTIFY Gene Family
TIFY is a plant-specific transcription factor that participates in the regulation of the division of plant meristems,which can reduce the damage caused by mechanical damage to plants,and can improve plant resistance to stress.In order to study the functions of the TIFY family related genes in B.napus,the homologous cloning method was used to clone the CDS(Coding sequence)of BnaA06g13250D in B.napus lines 1423 and 2205,at 12 and 24 h after NaHCO3 stress,the expression level of BnaA06g13250D gene in salt-tolerant line 2205 was significantly lower than that of salt-sensitive line 1423,and the salt tolerance of the two parent materials was significantly different.The results showed that the CDS of BnaJAZ1 was 762 bp,encoding 253 amino acids,and contained a TIFY domain;the Bna2205 cloned from the salt-tolerant variety 2205 had differences encoded by the BnaA06g13250D,there were 16 amino acid differences and one increase in amino acid sequence between Bna2205 and BnaA06g13250D gene of B.napus,and there were also differences in the primary and secondary structure of the protein.Phylogenetic analysis showed that the genetic distance between the BnaJAZ1 and B.nigra was the closest,but it was far from the species such as sweet potato.The study,we used blastp to identify and screen the whole genome of B.napus,and obtained 68 TIFY family genes,all of which contained TIFY conserved domains and three subfamilies,and there were significant differences in gene structure among the subfamilies,distributed on 19 chromosomes.This study laid a foundation for the research on the stress resistance of the TIFY family related genes,and provided a reference for the further study of alkali salt tolerance in B.napus.
Brassica napus L.Alkaline salt toleranceBnaJAZ1TIFY familyBioinformatics analysis
张琪、陶顺仙、苏倩、张燕、李萍、曹涵铭、Aldiyar Bakirov、徐爱遐、黄镇
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西北农林科技大学农学院,杨陵,712100
宝鸡市农业技术推广服务中心,宝鸡,721699
甘蓝型油菜(Brassica napus L.) 耐碱性盐 BnaJAZ1 TIFY家族 生物信息学分析
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