木薯MDH互作蛋白的筛选及验证
Screening and Identification of MeMDH Interacting Protein in Cassava
王冠琦 1白若宇 2周萌萌1
作者信息
- 1. 海南大学热带作物学院,海南省耐盐作物生物技术重点实验室,海口,570228
- 2. 海南大学热带作物学院,海南大学南繁与热带高效农业协同创新中心,海南省热带生物资源可持续利用重点实验室-省部共建国家重点实验室培育基地,海口,570228
- 折叠
摘要
苹果酸脱氢酶(malate dehydrogenase,MDH)在植物生长发育过程中发挥着至关重要的作用,然而在植物免疫中的作用,仍然是难以捉摸的.本研究在木薯中发现了一个MeMDH1基因,以MeMDH1为诱饵蛋白构建载体pGADT7-MeMDH1,从木薯cDNA文库筛选互作蛋白.得到一个候选基因MeLSD3,研究报道LSD在细胞程序性死亡以及植物光合作用和生长等生物过程中发挥重要作用.我们通过酵母双杂交(Yeast two-hybrid,Y2H)和双分子荧光互补(bimolecular fluorescence complementation,BiFC)实验验证了 MeLSD3 和MeMDH1在木薯体内体外的互作.为进一步探究木薯苹果酸脱氢酶在植物抗病反应中的功能及其调控的分子机制提供理论基础.
Abstract
Malate dehydrogenase(MDH)plays a crucial role in plant growth and development,yet its role in plant immunity remains elusive.In this study,a MeMDH1 gene was identified in cassava,and the vector pGADT7-MeMDH1 was constructed using MeMDH1 as a decoy protein to screen the reciprocal proteins from a cassava cDNA library.A candidate gene MeLSD3,was obtained and studies reported that LSD plays an important role in biological processes such as programmed cell death and plant photosynthesis and growth.We verified the interaction between MeLSD3 and MeMDH1 in cassava in vitro and in vivo by yeast two-hybrid(Y2H)and bi-molecular fluorescence complementation(BiFC)experiments.It provides a theoretical basis for further investiga-tion of the function of cassava malate dehydrogenase in plant disease resistance response and the molecular mecha-nism of its regulation.
关键词
木薯(Manihot/esculenta/Crantz)/苹果酸脱氢酶/酵母双杂交/互作蛋白/BiFCKey words
Cassava(Manihot esculenta Crantz)/Malate dehydrogenase/Yeast two-hybrid/Interacting protein/BiFC引用本文复制引用
基金项目
海南省科技专项重点研发计划项目(ZDYF2022XDNY139)
海南省科技条件平台专项研发支撑平台省重点实验室(RZ2300000259)
海南省研究生创新科研课题(Qhys2021-166)
出版年
2024
NETL
NSTL
万方数据