Transcriptome Data Analysis of Peucedanum praaeruptorum Dunn.Based on High-throughput Sequencing
The problem of early bolting in Peucedanum praaeruptorum Dunn.,and its bolting mechanism is still unclear.In this study,DNBSEQ second generation sequencing technology was used to conduct transcriptome se-quencing on the xylem and phloem of stems,leaves and roots of bolted and unbolted prohu,respectively,and bioinformatics analysis was conducted based on the sequencing results.A total of 51.21 Gb data were obtained by transcriptome sequencing analysis,and 178 764 unigenes were assembled by Trinity,which were compared and annotated with NR,Swiss-Port,KOG,KEGG,GO and other protein databases,and the results showed that P.praaeruptorum and Daucus carota subsp.sativus homologous sequences were the most,and 24 different KOG functional taxa were obtained by KOG functional classification analysis.A total of 818 428 unigenes are involved in 3 GO categories of biological processes(BP),cellular components(CC),and molecular functions(MF).KEGG annotated that there were a total of 22 752 differential genes between the leaves of underwent and did not undergo bolting.The genes related to premature bolting were screened out.The expression of the flowering integration fac-tor FT was up-regulated compared with that before pumping,the expression of CCA1 and ZTL was up-regulated,and the expression of FKF1 was down-regulated.The study of the DEGs between bolting and control group of P.praaeruptorum genes.Obtain the information characteristics of the transcriptome of P.praaeruptorum,and provide scientific basis for the subsequent research on the bolting mechanism of P.praaeruptorum and the mining of func-tional genes.
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万方数据
