Transcriptome Analysis of Imperata cylindrica by High-throughput Se-quencing and Gene Mining Related to Flavonoid Synthesis
Imperata cylindrica(L.)P.Beauv.to identify genes related to flavonoid synthesis.The results indicated that a total of 161 775 high-quality unigenes were obtained,with an N50 length of 969 bp and an average length of 145.86 bp.The percentage of Q20 bases ranged from 98.19％to 98.62％,while the percentage of Q30 bases varied between 94.05％and 95.28％.The transcripts were compared with seven major databases,including GO and KEGG,for functional annotation.It was found that 97 922 unigenes were annotated in at least one database,ac-counting for 60.53％of the total.There were 3 296 differentially expressed unigenes between the aboveground and underground parts of Imperata cylindrica,with 2 574 upregulated and 722 downregulated.The analysis of metabol-ic pathways in the KEGG database revealed that 12 enzymes are involved in the flavonoid biosynthesis pathway of Imperata cylindrica.The TMP values of key genes involved in flavonoid biosynthesis were compared between the aboveground and underground parts,revealing that 8 genes were highly expressed in the underground parts,and 4 genes were highly expressed in the aboveground parts(TMP＞15).This transcriptome sequencing effort has gener-ated a vast amount of gene sequences for Imperata cvlindrica,providing a theoretical basis for further research into the functional genes,biosynthesis regulation,and germplasm improvement for the medicinal active components of Imperata cylindrica.
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