Identification of Chinese Cabbage Seed Purity of'Jimei No.8'by KASP Markers
Seed purity is the core index of seed quality and the key factor to ensure the yield increase of excellent varieties.In this study,the Chinese Cabbage variety'Jimei No.8'and its parents were used as experimental materi-als,and the polymorphic primers between the hybrids and their parents were studied by KASP marker technology to identify the true and false hybrids.The results showed that six KASP markers with significant differences be-tween parents were selected from 26 KASP markers,namely KBr98,KBr100,KBr106,KBr109,KBr135 and KBr143.Three of them(KBr100,KBr106 and KBr143)were selected to analyze the purity of 136'Jimei 8'hybrids.The results showed that the three pairs of KASP markers identified the purity of'Jimei No.8'seeds were identical,and the purity of the hybrids was 96.32％.The purity of 136'Jimei No.8'hybrids was further analyzed by using In-Del marker BrID90039.The identification results were also consistent with the results of three KASP markers,which proved the accuracy of the identification results of KASP markers.Therefore,the six KASP markers select-ed in this study can be used for rapid identification of seed purity of Chinese cabbage hybrid'Jimei No.8'with low cost and high throughput.
Chinese cabbageJimei No.8KASP markerPurity identification
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NSTL
万方数据
