Genetic Diversity and Genetic Relationship of Salvia yunnanensis C.H.W.Wright Germplasm Resources by SSR Analysis
In order to explore the genetic relationship between Salvia yunnanensis C.H.W.Wright germplasm re-sources,simple sequence repeats(SSR)molecular markers were used to analyze the genetic diversity and genetic relationship of 269 materials from 4 species of Salvia plant.The results showed that the average of Shannon index(I)of269 materials from 4 populations was 0.368,the average expected heterozygosity(uHe)was 0.236,the average number of alleles(Na)was 1.757,the average effective number of alleles(Ne)was 1.365.The He of the Salvia yun-nanensis population was 0.280,which was higher than the average level of dicots(He=0.191),showing high genetic diversity.The He of the Salvia miltiorrhiza population(0.151)was lower than 0.191,indicating that the genetic diversity of the Salvia miltiorrhiza population was poor.40％of the genetic differentiation of 145 materials of the Salvia yunnanensis and Salvia miltiorrhiza Bage among the populations,and 60％exists within the populations,this showed that the genetic variation between populations was less than within populations.Using the method of calculating the best K value,cluster analysis was carried out on 269 materials,the maximum ΔK was K=2.Therefore,it can be judged that the 269 Salvia miltiorrhiza resources can be divided into two groups,but when ΔK is K=4,there is an obvious turning point,indicating that there is an exchange between the 269 Salvia resources.Through the development of EST-SSR molecular markers of Salvia yunnanensis and the analysis of genetic diversity,hybrid identification of F1 generation of Salvia yunnanensis can be carried out,and the molecular basis for genetic mapping of Salvia yunnanensis can be provided at the same time.
Salvia yunnanensis C.H.W.WrightSSR molecular markerGenetic diversityGenetic relationshipCluster analysis
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