Establishment and Application of RAPD-PCR System for Erythropalum scandens
To reveal the genetic variation of the Erythropalum scandens.In this study,the RAPD-PCR technology was used to analyze the genetic diversity of wild Erythropalum scandens varieties from 18 regions.Through orthogonal experiment and single factor experiment,the total volume of the RAPD-PCR amplification system is finally determined to be 25.0 μL,including 2.0 μL of primer;2.0 μL of DNA;12.5 μL of 2×Taq Master Mix.The optimized PCR amplification system was used to screen out 13 primers with good polymorphism and high repro-ducibility,with a total of 95 sites for amplification and 77 sites for polymorphism.When the genetic similarity coefficient is 0.71,it can be divided into 5 categories,which indicates that the genetic diversity of is Erythropalum scandens richer and there are large differences in the population.This research provides a theoretical basis for the selection and development of excellent varieties.
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