赤苍藤RAPD-PCR体系的建立与应用
Establishment and Application of RAPD-PCR System for Erythropalum scandens
赵昊天 1祝建波 1杨有兴 2谭骏 2文国荣 2王冬梅1
作者信息
- 1. 石河子大学生命科学学院,石河子,832003
- 2. 广西南宁市农业科学研究所,南宁,530021
- 折叠
摘要
为揭示赤苍藤的遗传变异情况,本研究采用RAPD-PCR技术对来自18个地区的野生赤苍藤品种进行了遗传多样性分析.通过正交试验和单因素试验确定RAPD-PCR扩增体系的总体积为25.0 μL,其中:引物2.0 μL;DNA2.0 μL;Master Mix 12.5 μL.使用优化后的PCR扩增体系筛选出13条多态性好重复性高的引物,共扩增位点95,多态性位点77,多态百分比为80.55％,遗传相似性系数在0.55～0.86之间,遗传距离范围为0.12～0.60,当遗传相似性系数为0.71时可分为5大类,表明赤苍藤遗传多样性较为丰富且种群内存在较大差异,为优异品种的筛选及开发提供理论基础.
Abstract
To reveal the genetic variation of the Erythropalum scandens.In this study,the RAPD-PCR technology was used to analyze the genetic diversity of wild Erythropalum scandens varieties from 18 regions.Through orthogonal experiment and single factor experiment,the total volume of the RAPD-PCR amplification system is finally determined to be 25.0 μL,including 2.0 μL of primer;2.0 μL of DNA;12.5 μL of 2×Taq Master Mix.The optimized PCR amplification system was used to screen out 13 primers with good polymorphism and high repro-ducibility,with a total of 95 sites for amplification and 77 sites for polymorphism.When the genetic similarity coefficient is 0.71,it can be divided into 5 categories,which indicates that the genetic diversity of is Erythropalum scandens richer and there are large differences in the population.This research provides a theoretical basis for the selection and development of excellent varieties.
关键词
赤苍藤/RAPD/遗传多样性Key words
Erythropalum scandens/RPAD/Genetic diversity引用本文复制引用
基金项目
广西壮族自治区科技厅重点研发计划项目(桂科AB19245042)
南宁市农业科学研究所重点科技专项(南农科项2020-3)
出版年
2024
NETL
NSTL
万方数据